发酵
纤维素酶
食品科学
马铃薯淀粉
黑曲霉
淀粉
食物垃圾
单细胞蛋白
羧甲基纤维素
拉伤
化学
水解
地衣芽孢杆菌
材料科学
生物化学
钠
废物管理
有机化学
生物
工程类
细菌
解剖
枯草芽孢杆菌
遗传学
作者
Bingnan Liu,Ying Li,Jinzhu Song,Lihong Zhang,Jiacheng Dong,Qian Yang
出处
期刊:Cellulose
[Springer Science+Business Media]
日期:2014-08-16
卷期号:21 (5): 3637-3645
被引量:33
标识
DOI:10.1007/s10570-014-0400-6
摘要
Potato starch processing waste is causing serious environmental problems. This study aimed to convert potato starch processing waste into single-cell protein as high-quality feed using a two-step fermentation process. The mutant strain Aspergillus niger H3 was selected after UV irradiation and ethyl methyl sulfone mutagenesis for more cellulase production. The activities of sodium carboxymethyl cellulase and filter paperase of strain H3 were 8.86 and 4.79 U, respectively, which were much higher than the parent strain (1.18 and 0.62 U). After treatment with strain H3, the cellulose degradation rate of potato residue was 80.54 %. A liquid fermentation using Bacillus licheniformis was performed as the second step. The optimized fermentation conditions were temperature of 32.8 °C, pH 6.67, and inoculum concentration of 1.78 % using the response surface method. Results of this study showed a potential application in large-scale industrial conversion.
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