Effect of metformin on bone marrow progenitor cell differentiation: In vivo and in vitro studies

二甲双胍 内分泌学 内科学 运行x2 体内 安普克 骨髓 祖细胞 骨钙素 成骨细胞 医学 糖尿病 生物 体外 干细胞 碱性磷酸酶 细胞生物学 蛋白激酶A 生物化学 生物技术 磷酸化
作者
María Silvina Molinuevo,León Schurman,Antonio Desmond McCarthy,Ana Marı́a Cortizo,María José Tolosa,María Virginia Gangoiti,Verónica Arnol,Claudia Sedlinsky
出处
期刊:Journal of Bone and Mineral Research [Oxford University Press]
卷期号:25 (2): 211-221 被引量:221
标识
DOI:10.1359/jbmr.090732
摘要

Abstract Diabetes mellitus is associated with bone loss. Patients with type 2 diabetes are frequently treated with oral antidiabetic drugs such as sulfonylureas, biguanides, and thiazolidinediones. Rosiglitazone treatment has been shown to increase adipogenesis in bone marrow and to induce bone loss. In this study we evaluated the effect of in vivo and in vitro treatment with metformin on bone marrow progenitor cells (BMPCs), as well as the involvement of AMPK pathway in its effects. The in vitro effect of coincubation with metformin and rosiglitazone on the adipogenic differentiation of BMPCs also was studied. In addition, we evaluated the effect of in vivo metformin treatment on bone regeneration in a model of parietal lesions in nondiabetic and streptozotocin-induced diabetic rats. We found that metformin administration both in vivo and in vitro caused an increase in alkaline phosphatase activity, type I collagen synthesis, osteocalcin expression, and extracellular calcium deposition of BMPCs. Moreover, metformin significantly activated AMPK in undifferentiated BMPCs. In vivo, metformin administration enhanced the expression of osteoblast-specific transcription factor Runx2/Cbfa1 and activation of AMPK in a time-dependent manner. Metformin treatment also stimulated bone lesion regeneration in control and diabetic rats. In vitro, metformin partially inhibited the adipogenic actions of rosiglitazone on BMPCs. In conclusion, our results indicate that metformin causes an osteogenic effect both in vivo and in vitro, possibly mediated by Runx2/Cbfa1 and AMPK activation, suggesting a possible action of metformin in a shift toward the osteoblastic differentiation of BMPCs. © 2010 American Society for Bone and Mineral Research
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