丝素
脚手架
去细胞化
材料科学
生物复合材料
组织工程
生物相容性
生物医学工程
细胞外基质
再生(生物学)
生物高聚物
骨组织
纳米纤维
丝绸
纳米技术
化学
复合材料
细胞生物学
复合数
聚合物
冶金
生物
医学
生物化学
作者
Hyeongjin Lee,Gi Hoon Yang,Minseong Kim,JaeYoon Lee,JunTae Huh,GeunHyung Kim
标识
DOI:10.1016/j.msec.2017.11.013
摘要
Biomaterials must be biocompatible, biodegradable, and mechanically stable to be used for tissue engineering applications. Among various biomaterials, a natural-based biopolymer, collagen, has been widely applied in tissue engineering because of its outstanding biocompatibility. However, due to its low mechanical properties, collagen has been a challenge to build a desired/complex 3D porous structure with appropriate mechanical strength. To overcome this problem, in this study, we used a low temperature printing process to create a 3D porous scaffold consisting of collagen, decellularized extracellular matrix (dECM) to induce high cellular activities, and silk-fibroin (SF) to attain the proper mechanical strength. To show the feasibility of the scaffold, pre-osteoblast (MC3T3-E1) cells were grown on the fabricated scaffold. Various in vitro cellular activities (cell-viability, MTT assay, and osteogenic activity) for pure collagen, collagen/dECM, and collagen/SF/dECM scaffolds were compared.
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