Human Gut Microbiome: Function Matters

生物 微生物群 基因组 计算生物学 功能(生物学) 肠道微生物群 人体微生物群 寄主(生物学) 数据科学 生态学 进化生物学 生物信息学 基因 遗传学 计算机科学
作者
Anna Heintz‐Buschart,Paul Wilmes
出处
期刊:Trends in Microbiology [Elsevier]
卷期号:26 (7): 563-574 被引量:563
标识
DOI:10.1016/j.tim.2017.11.002
摘要

Functional omics are becoming more accessible, and increasing numbers of studies have employed them, demonstrating their potential in identifying functional traits of the microbiome related to health and disease. Functional omes display greater variability and sensitivity to perturbation, also in cases of where changes in taxonomic composition are minimal, and they can resolve gut-compartment-specific information. Methods for resolving functional differences in meta-omic datasets to the taxa contributing them have been developed and are necessary to understand the impact of microbial functions on human physiology. The human gut microbiome represents a complex ecosystem contributing essential functions to its host. Recent large-scale metagenomic studies have provided insights into its structure and functional potential. However, the functional repertoire which is actually contributed to human physiology remains largely unexplored. Here, by leveraging recent omics datasets, we challenge current assumptions regarding key attributes of the functional gut microbiome, in particular with respect to its variability. We further argue that the closing of existing gaps in functional knowledge should be addressed by a most-wanted gene list, the development and application of molecular and cellular high-throughput measurements, the development and sensible use of experimental models, as well as the direct study of observable molecular effects in the human host. The human gut microbiome represents a complex ecosystem contributing essential functions to its host. Recent large-scale metagenomic studies have provided insights into its structure and functional potential. However, the functional repertoire which is actually contributed to human physiology remains largely unexplored. Here, by leveraging recent omics datasets, we challenge current assumptions regarding key attributes of the functional gut microbiome, in particular with respect to its variability. We further argue that the closing of existing gaps in functional knowledge should be addressed by a most-wanted gene list, the development and application of molecular and cellular high-throughput measurements, the development and sensible use of experimental models, as well as the direct study of observable molecular effects in the human host. systems that can be employed as a model for the human microbiome and which are amenable to manipulation; they range from mixed-species and automated culturing, cell-culture-based coculture systems, and, as all animals carry a microbiome, animal models, including germ-free or gnotobiotic animals, including those with a ‘humanized’ microbiome. are commonly applied to describe the functions encoded or carried out by the microbiome (the functional complement), usually by grouping genes into metabolism-centered frameworks, such as the orthology put forth by the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the MetaCyc database, or functionally annotated orthologous groups based on sequence similarity, such as eggNOG. is a measure of the number (richness) and distribution of different functions within the community. It is related to gene richness but also to phylogenetic diversity, as microbial communities with phylogenetically diverse members often have a wider functional potential. Phylogenetic diversity and functional diversity have been observed as traits of human gut microbiota which are relatively stable over time. the ability of the microbial community or its members to adapt to perturbations by changing gene expression; it can stabilize the taxonomic community structure as well as ecosystem functions. is a measure of the number of different populations within a community that are able to perform the same functions. Functional redundancy can increase functional resilience, in case perturbations affect the taxonomic community structure; this allows for a return to community function, and therefore can increase stability. seek to manipulate the human microbiome in situ, by means of nutrition, probiotics, antibiotics, or faecal transplants. refers to the analysis of genomic DNA for mixtures of (often unknown) species. Its purpose can be to assess the taxonomic composition of a mixed microbial community or to elucidate the functional potential of its members. (also referred to as metabonomics, mainly in the context of research on single organisms) technologies that measure intra- and/or extracellular metabolites in and around microbial communities. aims to characterize microbial activity by applying the analysis of proteomes to mixed-species assemblages. is the term applied to the analysis of RNA of communities, usually with the aim of inferring activity. a group of methodologies that aim at the characterization of the total pool of a class of biomolecules, including metagenomics, (meta)metabolomics, metaproteomics, and metatranscriptomics. to quantify the taxa and functions detected in a sample form part of most meta-omic studies of the human microbiome. Increasingly, taxonomic resolution of functions of interest within the microbiome is also achieved.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
修猫完成签到 ,获得积分10
刚刚
CHEN.CHENG完成签到,获得积分20
刚刚
积极的尔白完成签到 ,获得积分10
刚刚
z_king_d_23完成签到,获得积分10
2秒前
kzhao发布了新的文献求助10
2秒前
糟糕的铃铛完成签到,获得积分10
3秒前
3秒前
chen完成签到,获得积分10
3秒前
清爽的冰凡完成签到 ,获得积分10
4秒前
feimengxia完成签到 ,获得积分10
4秒前
Lucy小影完成签到,获得积分10
4秒前
Accepted应助winwin采纳,获得10
5秒前
5秒前
悲凉的孤萍完成签到,获得积分10
5秒前
赘婿应助黑粉头头采纳,获得10
6秒前
volcano完成签到 ,获得积分10
6秒前
花样年华完成签到,获得积分0
7秒前
我劝告了风完成签到,获得积分10
7秒前
qingli完成签到,获得积分10
8秒前
9秒前
打打应助kzhao采纳,获得10
9秒前
9秒前
9秒前
10秒前
10秒前
共享精神应助萝卜特二采纳,获得10
10秒前
领导范儿应助我劝告了风采纳,获得10
11秒前
搬石头完成签到,获得积分10
11秒前
荆轲刺秦王完成签到 ,获得积分10
12秒前
CDH完成签到,获得积分10
12秒前
木香完成签到,获得积分10
13秒前
新青年应助77采纳,获得10
13秒前
自觉大碗完成签到,获得积分10
13秒前
wenming完成签到,获得积分10
14秒前
哇哇哇完成签到 ,获得积分10
14秒前
四斤瓜完成签到 ,获得积分10
14秒前
虚幻的海安完成签到,获得积分10
14秒前
14秒前
tesla发布了新的文献求助10
14秒前
15秒前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3099877
求助须知:如何正确求助?哪些是违规求助? 2751333
关于积分的说明 7612942
捐赠科研通 2403282
什么是DOI,文献DOI怎么找? 1275217
科研通“疑难数据库(出版商)”最低求助积分说明 616310
版权声明 599053