荧光
头孢噻肟
细菌
部分
化学
抗生素耐药性
抗菌剂
致病菌
选择性
试剂
组合化学
头孢菌素
抗生素
微生物学
生物物理学
生物化学
生物
立体化学
催化作用
有机化学
遗传学
量子力学
物理
作者
Wuyu Mao,Xiana Qian,Jian Zhang,Lingying Xia,Hexin Xie
出处
期刊:ChemBioChem
[Wiley]
日期:2017-08-23
卷期号:18 (20): 1990-1994
被引量:20
标识
DOI:10.1002/cbic.201700447
摘要
Abstract The dissemination of antimicrobial resistance around the world is one of the biggest threats to global public health. The acquisition and expression of extended‐spectrum β‐lactamases (ESBLs) in pathogenic bacterial are mainly responsible for bacterial resistance to third‐generation cephalosporins. Reported herein is a ratiometric fluorescent probe for the detection of the activity of ESBLs. This imaging reagent adopts the core structure of cefotaxime as an enzymatic recognition moiety, and exhibits excellent selectivity to ESBLs over other β‐lactamases. The specific activation of this sensor by ESBLs can lead to over 2500‐fold changes in the fluorescent ratio, which is independent of the concentration of the probe and environmental conditions. Further experiments have demonstrated that this ratiometric fluorescent probe can distinguish bacteria with extended‐spectrum antibiotic resistance from a group of clinically important pathogens within a short period of time.
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