Usnea species are medicinally important, so we take unexplored species, Usnea emidotteris. Seven components 2-hydroxy-3-methoxy-4, 6-dimethyl ethyl benzoate, 2, 4-dihydro-xy-3, 6-dimethyl ethyl benzoate, 2-hydroxy-4-methoxy-3, 6-dimethylbezoic acid, usnic acid, barbatic acid, diffractaic acid and evernic acid are isolated first time from Usnea emidotteries and identified from their physicochemical data.