Objective To measure uric acid of seminal plasma in fertile and infertile males. Methods One hundred and sixty-three infertile males were divided into an obstructive azoospermic group (15 cases), a non-obstructive azoospermic group (36 cases), an oligozoospermic group (43 cases), and an asthenozoospermic group (69 cases). Twenty fertile males were included in the control group. Uric acid concentrations of seminal plasma in the fertile and infertile men were assessed by spectrophotometer, and sperm parameters were analyzed by computer-assisted semen analysis (CASA) system. Results Uric acid concentration of seminal plasma in the control group was significantly higher than all the infertile groups (P 0.05). Conclusion Uric acid may play an important role in male reproduction because of its antioxidative property.