P19电池
生物
细胞分化
畸胎瘤
细胞培养
细胞凋亡
细胞生物学
细胞生长
分子生物学
二甲基亚砜
免疫学
生物化学
化学
成体干细胞
遗传学
基因
有机化学
作者
Jasmin Jasmin,David C. Spray,Antônio Carlos Campos de Carvalho,Rosalia Méndez‐Otero
出处
期刊:Stem Cells and Development
[Mary Ann Liebert]
日期:2010-03-01
卷期号:19 (3): 403-412
被引量:45
标识
DOI:10.1089/scd.2009.0234
摘要
P19 cells, a pluripotent cell line derived from a teratocarcinoma induced in C3H/HeHa mice, have been widely used as a model system to study cardiac differentiation. We have used these cells to evaluate the extent to which exposure to DMSO and/or cardiogenol C for 4 days in suspension culture enhanced their differentiation into cardiomyocytes. Cardiac differentiation was assessed by observing beating clusters and further confirmed using immunocytochemical, biochemical, and pharmacological approaches. The presence of functional gap junctions in differentiated P19 cells was identified through calcium wave analyses. Proliferation rate and cell death were analyzed by BrdU incorporation and activated caspase-3 immunodetection, respectively. Beating clusters of differentiated P19 cells were only found in cultures treated with DMSO. In addition, groups treated with DMSO up-regulated cardiac troponin-T expression. However, when DMSO was used together with cardiogenol C the up-regulation was less than that with DMSO alone, approximately 1.5 times. Moreover, P19 cells cultured in DMSO or DMSO plus 0.25 microM cardiogenol C had lower proliferation rates and higher numbers of activated caspase-3-positive cells. In summary, using several methodological approaches we have demonstrated that DMSO can induce cardiac differentiation of P19 cells but that cardiogenol C does not.
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