Identification of Genes in Thuja plicata Foliar Terpenoid Defenses

萨比宁 生物 萜类 转录组 基因 候选基因 单萜 植物 图哈 基因表达 遗传学 柠檬烯 精油
作者
Adam Foster,Dawn E. Hall,Leanne Mortimer,Shelley Abercromby,Regine Gries,Gerhard Gries,Jörg Bohlmann,John H. Russell,Jim Mattsson
出处
期刊:Plant Physiology [Oxford University Press]
卷期号:161 (4): 1993-2004 被引量:29
标识
DOI:10.1104/pp.112.206383
摘要

Abstract Thuja plicata (western redcedar) is a long-lived conifer species whose foliage is rarely affected by disease or insect pests, but can be severely damaged by ungulate browsing. Deterrence to browsing correlates with high foliar levels of terpenoids, in particular the monoterpenoid α-thujone. Here, we set out to identify genes whose products may be involved in the production of α-thujone and other terpenoids in this species. First, we generated a foliar transcriptome database from which to draw candidate genes. Second, we mapped the storage of thujones and other terpenoids to foliar glands. Third, we used global expression profiling to identify more than 600 genes that are expressed at high levels in foliage with glands, but can either not be detected or are expressed at low levels in a natural variant lacking foliar glands. Fourth, we used in situ RNA hybridization to map the expression of a putative monoterpene synthase to the epithelium of glands and used enzyme assays with recombinant protein of the same gene to show that it produces sabinene, the monoterpene precursor of α-thujone. Finally, we identified candidate genes with predicted enzymatic functions for the conversion of sabinene to α-thujone. Taken together, this approach generated both general resources and detailed functional characterization in the identification of genes of foliar terpenoid biosynthesis in T. plicata.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小向爱科研完成签到,获得积分10
2秒前
Yuhong完成签到,获得积分10
4秒前
脑洞疼应助AAA电材哥采纳,获得10
6秒前
7秒前
阔达的无剑应助张宝采纳,获得10
7秒前
大胆的渊思完成签到 ,获得积分10
9秒前
香蕉觅云应助冯兴龙采纳,获得10
10秒前
10秒前
11秒前
12秒前
Uuuuuuumi发布了新的文献求助10
12秒前
13秒前
14秒前
15秒前
15秒前
hushan53发布了新的文献求助30
16秒前
元友容完成签到 ,获得积分10
17秒前
17秒前
苗条以南完成签到,获得积分10
17秒前
AAA电材哥发布了新的文献求助10
17秒前
加菲丰丰应助SYX采纳,获得20
18秒前
Quitter发布了新的文献求助10
18秒前
爆米花应助精明晓刚采纳,获得10
18秒前
18秒前
斯文败类应助rush采纳,获得30
19秒前
xiaoq发布了新的文献求助10
19秒前
CodeCraft应助王泽同采纳,获得10
20秒前
20秒前
Fan完成签到,获得积分10
20秒前
22秒前
噜啦啦完成签到,获得积分10
22秒前
AAA电材哥完成签到,获得积分10
22秒前
23秒前
暮光之城完成签到,获得积分10
23秒前
carrier_hc完成签到,获得积分10
23秒前
冯兴龙发布了新的文献求助10
23秒前
Owen应助泡面公主采纳,获得10
25秒前
26秒前
噜啦啦发布了新的文献求助10
27秒前
30秒前
高分求助中
Evolution 10000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
The Kinetic Nitration and Basicity of 1,2,4-Triazol-5-ones 440
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3164351
求助须知:如何正确求助?哪些是违规求助? 2815193
关于积分的说明 7908079
捐赠科研通 2474802
什么是DOI,文献DOI怎么找? 1317676
科研通“疑难数据库(出版商)”最低求助积分说明 631925
版权声明 602234