Acute hypoxia and osteoclast activity: a balance between enhanced resorption and increased apoptosis

破骨细胞 骨吸收 吸收 组织蛋白酶K 内分泌学 细胞凋亡 组织蛋白酶 内科学 台盼蓝 化学 缺氧(环境) 酸性磷酸酶 标记法 生物 医学 生物化学 受体 有机化学 氧气
作者
Helen J. Knowles,N. A. Athanasou
标识
DOI:10.1002/path.2534
摘要

Abstract Osteoclasts are the primary mediators of pathological bone resorption in many conditions in which micro‐environmental hypoxia is associated with disease progression. However, effects of hypoxia on human osteoclast activity have not been reported. Mature human osteoclasts were differentiated from peripheral blood or obtained from giant cell tumour of bone. Osteoclasts were exposed to a constant hypoxic environment and then assessed for parameters including resorption (toluidine blue staining of dentine slices), membrane integrity (trypan blue exclusion), apoptosis (TUNEL, DAPI), and osteolysis‐associated enzyme activity (TRAP, cathepsin K). 24 h exposure to 2% O 2 produced a 2.5‐fold increase in resorption associated with increased TRAP and cathepsin K enzyme activity. Hypoxia‐Inducible Factor‐1α (HIF‐1α) siRNA completely ablated the hypoxic increase in osteoclast resorption. 24 h at 2% O 2 also increased the number of osteoclasts with compromised membrane integrity from 6% to 21%, with no change in the total osteoclast number or the proportion of late‐stage apoptotic cells. Transient reoxygenation returned the percentage of trypan blue‐positive cells to normoxic levels, suggesting that osteoclasts can recover from the early stages of cell death. Repeated over an extended period, hypoxia/reoxygenation enhanced osteoclast differentiation at this p O 2 . These data suggest that in diseased bone, where the p O 2 may fall to ≤2% O 2 , a delicate balance between hypoxia‐induced osteoclast activation and hypoxia‐induced osteoclast apoptosis mediates pathological bone resorption. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
机灵夏云发布了新的文献求助10
刚刚
灵巧剑心完成签到,获得积分20
3秒前
花海完成签到,获得积分10
3秒前
4秒前
虞头星星发布了新的文献求助10
5秒前
6秒前
lf发布了新的文献求助10
8秒前
iNk应助小洪俊熙采纳,获得50
8秒前
meng完成签到,获得积分10
9秒前
慕青应助机灵夏云采纳,获得10
14秒前
15秒前
嘉心糖应助teargasxq采纳,获得30
16秒前
20秒前
21秒前
静默发布了新的文献求助10
21秒前
ding应助Ding-Ding采纳,获得10
21秒前
源S发布了新的文献求助10
23秒前
23秒前
24秒前
Justtry发布了新的文献求助10
25秒前
SciGPT应助yangwenjie1212采纳,获得10
28秒前
28秒前
29秒前
35秒前
科研通AI6.2应助wilson采纳,获得10
35秒前
摩西不晚完成签到,获得积分10
37秒前
meng发布了新的文献求助10
38秒前
41秒前
研友_8op0RL完成签到,获得积分10
41秒前
42秒前
44秒前
摩西不晚发布了新的文献求助10
45秒前
yana发布了新的文献求助20
45秒前
46秒前
keke完成签到 ,获得积分10
46秒前
蒲公英完成签到,获得积分10
47秒前
爆米花应助M__M采纳,获得10
47秒前
Sora发布了新的文献求助10
49秒前
52秒前
Heart完成签到,获得积分10
52秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Developing Genetic Editing Tools for Lysobacter 2000
卤化钙钛矿人工突触的研究 2000
Моделирование процессов самоорганизации в кристаллообразующих системах 1000
History of U.S. Space Surveillance and Satellite Cataloging 1000
Malcolm Fraser : a biography 700
Handbook of Optical Systems,Volume 6:Advanced Physical Optics 666
热门求助领域 (近24小时)
化学 材料科学 医学 生物 纳米技术 工程类 有机化学 化学工程 生物化学 计算机科学 物理 内科学 复合材料 催化作用 物理化学 光电子学 电极 细胞生物学 基因 无机化学
热门帖子
关注 科研通微信公众号,转发送积分 6514548
求助须知:如何正确求助?哪些是违规求助? 8308004
关于积分的说明 17753889
捐赠科研通 5616406
什么是DOI,文献DOI怎么找? 2924675
邀请新用户注册赠送积分活动 1901661
关于科研通互助平台的介绍 1763068