叶绿素
分解代谢
西葫芦
生物
生物化学
原生质体
光合作用
烟草
叶绿体
酶
南瓜
植物
基因
作者
Smadar Harpaz‐Saad,Tamar Azoulay,Tzahi Arazi,Eran Ben-Yaakov,Anahit Mett,Yoel Moshe Shiboleth,Stefan Hörtensteiner,David Gidoni,Amit Gal‐On,Eliezer Ε. Goldschmidt,Yoram Eyal
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2007-03-01
卷期号:19 (3): 1007-1022
被引量:231
标识
DOI:10.1105/tpc.107.050633
摘要
Chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. To study the role and regulation of chlorophyllase (Chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (Citrus sinensis) Chlase in two heterologous plant systems: (1) squash (Cucurbita pepo) plants using a viral vector expression system; and (2) transiently transformed tobacco (Nicotiana tabacum) protoplasts. Expression of full-length citrus Chlase resulted in limited chlorophyll breakdown in protoplasts and no visible leaf phenotype in whole plants, whereas expression of a Chlase version lacking the N-terminal 21 amino acids (ChlaseΔN), which corresponds to the mature protein, led to extensive chlorophyll breakdown in both tobacco protoplasts and squash leaves. ChlaseΔN-expressing squash leaves displayed a dramatic chlorotic phenotype in plants grown under low-intensity light, whereas under natural light a lesion-mimic phenotype occurred, which was demonstrated to follow the accumulation of chlorophyllide, a photodynamic chlorophyll breakdown product. Full-length and mature citrus Chlase versions were localized to the chloroplast membrane fraction in expressing tobacco protoplasts, where processing of the N-terminal 21 amino acids appears to occur. Results obtained in both plant systems suggest that Chlase functions as a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
科研通智能强力驱动
Strongly Powered by AbleSci AI