Characterisation of an (S)-linalool synthase from kiwifruit (Actinidia arguta) that catalyses the first committed step in the production of floral lilac compounds

芳樟醇 丁香花 花瓣 生物 植物 猕猴桃 萜烯 单萜 园艺 生物化学 精油
作者
Xiuyin Chen,Yar‐Khing Yauk,Niels J. Nieuwenhuizen,Adam J. Matich,Mindy Wang,Ramon Lopez Perez,Ross G. Atkinson,Lesley L. Beuning
出处
期刊:Functional Plant Biology [CSIRO Publishing]
卷期号:37 (3): 232-232 被引量:40
标识
DOI:10.1071/fp09179
摘要

Kiwifruit (Actinidia spp. Lindl.) flowers and fruit contain many compounds of interest to the flavour and fragrance industries. In particular, Actinidia arguta (Sieb. et Zucc.) Planch. ex Miq. flowers produce β-linalool and important derivatives thereof, including linalool oxides, lilac aldehydes, alcohols and alcohol epoxides. Dynamic headspace sampling of whole A. arguta flowers showed that the peak emission rate of linalool, lilac alcohols and lilac aldehydes occurred around 0800 hours. After solvent extraction, linalool levels remained constant throughout the day and night, but lilac alcohol levels peaked at noon. In whole flowers, linalool was found predominantly in pistils and petals, and the lilac compounds were found mainly in petals. Two highly homologous (96.6% nucleotide identity) terpene synthase cDNA sequences, AaLS1 and ApLS1, were isolated from A. arguta and Actinidia polygama (Sieb. et Zucc.) Maxim flower EST libraries respectively. Real-time PCR analysis revealed that AaLS1 was expressed constitutively throughout the day and night, and primarily in petal tissue. Functional analysis in Escherichia coli showed that AaLS1 and ApLS1 each encoded a linalool synthase which was confirmed by transient expression in planta. Enantioselective gas chromatography revealed that both terpene synthases produced only (S)-(+)-linalool. AaLS1, therefore, is likely to be the key enzyme producing the (S)-linalool precursor of the lilac alcohols and aldehydes in A. arguta flowers.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
耿春丽完成签到,获得积分10
刚刚
刚刚
2秒前
weilu发布了新的文献求助10
2秒前
3秒前
嘟嘟发布了新的文献求助10
4秒前
陈开山完成签到,获得积分10
4秒前
梨理栗发布了新的文献求助10
4秒前
4秒前
alex发布了新的文献求助10
5秒前
5秒前
LaTeXer应助隐形夕阳采纳,获得50
5秒前
lw发布了新的文献求助10
6秒前
6秒前
英姑应助Janusfaces采纳,获得10
6秒前
6秒前
plasmid完成签到,获得积分10
7秒前
Ava应助咕噜咕噜咕嘟咕嘟采纳,获得10
7秒前
8秒前
SHAO应助一块司康饼采纳,获得100
8秒前
嗯哼发布了新的文献求助10
8秒前
Rondab应助mariawang采纳,获得10
10秒前
MchemG应助酷酷的紫南采纳,获得30
11秒前
1111发布了新的文献求助10
11秒前
11秒前
continue发布了新的文献求助10
12秒前
zhangtong发布了新的文献求助10
12秒前
嘟嘟完成签到,获得积分10
12秒前
wdy111应助葡萄味的果茶采纳,获得20
13秒前
悦耳代真完成签到,获得积分10
13秒前
ysx完成签到,获得积分10
13秒前
14秒前
Orange应助淡淡夕阳采纳,获得10
14秒前
14秒前
yar重新开启了yl文献应助
15秒前
16秒前
16秒前
zhoup完成签到,获得积分20
17秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988732
求助须知:如何正确求助?哪些是违规求助? 3531027
关于积分的说明 11252281
捐赠科研通 3269732
什么是DOI,文献DOI怎么找? 1804764
邀请新用户注册赠送积分活动 881869
科研通“疑难数据库(出版商)”最低求助积分说明 809021