CD86
CD28
CD80
中国仓鼠卵巢细胞
细胞生物学
T细胞
生物
分子生物学
转染
细胞毒性T细胞
CD40
细胞培养
免疫系统
免疫学
体外
生物化学
遗传学
作者
Per Höllsberg,Christian Schölz,David E. Anderson,Edward Greenfield,Vijay K. Kuchroo,G J Freeman,D A Hafler
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1997-11-15
卷期号:159 (10): 4799-4805
被引量:63
标识
DOI:10.4049/jimmunol.159.10.4799
摘要
CD80 (B7-1) and CD86 (B7-2) on APC provide a major costimulatory signal through interactions with CD28 on T cells. Absent from resting human T cells, CD86 is up-regulated early upon T cell activation, whereas CD80 expression appears later. Whereas T cell expression of CD80 has been implicated in costimulation, the functional significance of CD86 expression on T cells is unclear. We now demonstrate that CD86 expressed on human CD4+ T cell clones does not provide a costimulatory signal for other CD4+ T cell clones. Binding studies using CD28-Ig and CTLA-4-Ig fusion proteins demonstrate that CD86 expressed on T cells has significantly reduced binding affinity for CTLA-4 and no detectable binding to CD28. Biochemical analysis demonstrates that post-translational modifications of CD86 in human T cells are different from those of CD86-transfected Chinese hamster ovary cells or EBV-transformed B cells, in that T cells express a hypoglycosylated form of CD86 on the surface membrane. Thus, our results suggest that while CD86 is expressed on a number of different cell types, its costimulatory function and affinity for its ligands may be regulated by cell type-specific post-translational modifications.
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