The rice phosphoinositide-specific phospholipase C3 is involved in responses to osmotic stresses via modulating ROS homeostasis

甘露醇 生物 渗透性休克 渗透 渗透调节 脯氨酸 磷脂酶C 突变体 活性氧 生物化学 细胞生物学 渗透压 磷脂酶 平衡 渗透压 渗透浓度 基因 盐度 氨基酸 生态学
作者
Min Yu,Chunyan Cao,Xiaoming Yin,Xiong Liu,Di Yang,Chunyan Gong,Hengtao Wang,Yan Wu
出处
期刊:Plant Science [Elsevier]
卷期号:313: 111087-111087 被引量:8
标识
DOI:10.1016/j.plantsci.2021.111087
摘要

Four members of phosphoinositide-specific phospholipase C (PI-PLC) are predicted in rice genome. Although the involvement of OsPLC1 and OsPLC4 in the responses of rice to salt and drought stresses has been documented, the role of OsPLC3 in which, yet, is elusive. Here, we report that OsPLC3 was ubiquitously expressed in various tissues during the development of rice. The expression of YFP-tagged OsPLC3 was observed at the plasma membrane (PM), cytoplasm and nucleus of rice protoplasts, onion epidermal cells and tobacco leaves. The catalytic activity of OsPLC3 was measured using the thin-layer chromatography (TLC) method. The inhibition of OsPLC3 expression was detected in the treatments of NaCl and mannitol. Overexpression (OE) of OsPLC3 produced plants showing more sensitive to osmotic stresses when they were compared to the wild-type (HJ) and osplc3 mutants, the phenomena such as decreased plant fresh weight and increased water loss rate (WLR) were observed. Under the treatment of NaCl or mannitol, expressions of a subset osmotic stress-related genes were altered, in both OE and osplc3 mutant lines. In addition, the expressions and the enzyme activities of reactive oxygen species (ROS) scavengers were significantly decreased in OE lines, leading to over-accumulation of ROS together with less osmotic adjustment substances including proline, soluble sugars and soluble proteins in OE plants which caused the growth inhibition. Thus, our results suggested that, via modulating ROS homeostasis, OsPLC3 is involved in responses to the osmotic stress in rice.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
传奇3应助tfq200采纳,获得10
刚刚
1秒前
348847119完成签到,获得积分10
4秒前
酷波er应助美好的大白采纳,获得10
4秒前
tuzhihong发布了新的文献求助10
5秒前
研猫发布了新的文献求助10
5秒前
5秒前
小狗发布了新的文献求助10
5秒前
JZJ完成签到,获得积分20
6秒前
Yan完成签到,获得积分10
7秒前
乐乐应助YaGue采纳,获得10
7秒前
动人的寒风完成签到,获得积分10
8秒前
公冶友儿完成签到,获得积分10
8秒前
9秒前
怕孤单的思雁完成签到,获得积分10
10秒前
顾矜应助等待的谷波采纳,获得10
11秒前
chjy发布了新的文献求助10
11秒前
11秒前
去看星星呀完成签到,获得积分10
12秒前
Akim应助Kaolala采纳,获得10
12秒前
12秒前
13秒前
梨炒栗子完成签到,获得积分10
13秒前
可爱的函函应助十七采纳,获得10
14秒前
nn666发布了新的文献求助10
14秒前
14秒前
Tina泽发布了新的文献求助10
14秒前
15秒前
乐乐应助李哈哈采纳,获得10
15秒前
18秒前
18秒前
19秒前
ladder发布了新的文献求助10
19秒前
20秒前
Tina泽完成签到,获得积分10
20秒前
21秒前
22秒前
踏实的便当完成签到,获得积分10
22秒前
小马甲应助火星上的听云采纳,获得30
22秒前
wjm发布了新的文献求助30
23秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
Sustainability in ’Tides Chemistry 1500
Studien zur Ideengeschichte der Gesetzgebung 1000
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3071903
求助须知:如何正确求助?哪些是违规求助? 2725788
关于积分的说明 7491264
捐赠科研通 2373147
什么是DOI,文献DOI怎么找? 1258476
科研通“疑难数据库(出版商)”最低求助积分说明 610277
版权声明 596944