已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

The rice phosphoinositide-specific phospholipase C3 is involved in responses to osmotic stresses via modulating ROS homeostasis

甘露醇 生物 渗透性休克 渗透 渗透调节 脯氨酸 磷脂酶C 突变体 活性氧 生物化学 细胞生物学 渗透压 磷脂酶 平衡 渗透压 渗透浓度 基因 盐度 氨基酸 生态学
作者
Min Yu,Chunyan Cao,Xiaoming Yin,Xiong Liu,Di Yang,Chunyan Gong,Hengtao Wang,Yan Wu
出处
期刊:Plant Science [Elsevier]
卷期号:313: 111087-111087 被引量:8
标识
DOI:10.1016/j.plantsci.2021.111087
摘要

Four members of phosphoinositide-specific phospholipase C (PI-PLC) are predicted in rice genome. Although the involvement of OsPLC1 and OsPLC4 in the responses of rice to salt and drought stresses has been documented, the role of OsPLC3 in which, yet, is elusive. Here, we report that OsPLC3 was ubiquitously expressed in various tissues during the development of rice. The expression of YFP-tagged OsPLC3 was observed at the plasma membrane (PM), cytoplasm and nucleus of rice protoplasts, onion epidermal cells and tobacco leaves. The catalytic activity of OsPLC3 was measured using the thin-layer chromatography (TLC) method. The inhibition of OsPLC3 expression was detected in the treatments of NaCl and mannitol. Overexpression (OE) of OsPLC3 produced plants showing more sensitive to osmotic stresses when they were compared to the wild-type (HJ) and osplc3 mutants, the phenomena such as decreased plant fresh weight and increased water loss rate (WLR) were observed. Under the treatment of NaCl or mannitol, expressions of a subset osmotic stress-related genes were altered, in both OE and osplc3 mutant lines. In addition, the expressions and the enzyme activities of reactive oxygen species (ROS) scavengers were significantly decreased in OE lines, leading to over-accumulation of ROS together with less osmotic adjustment substances including proline, soluble sugars and soluble proteins in OE plants which caused the growth inhibition. Thus, our results suggested that, via modulating ROS homeostasis, OsPLC3 is involved in responses to the osmotic stress in rice.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
人小鸭儿大完成签到 ,获得积分10
1秒前
3秒前
科研通AI2S应助科研通管家采纳,获得10
4秒前
共享精神应助科研通管家采纳,获得30
4秒前
赘婿应助科研通管家采纳,获得10
4秒前
脑洞疼应助科研通管家采纳,获得10
4秒前
浅尝离白应助科研通管家采纳,获得10
5秒前
5秒前
Allen完成签到,获得积分10
5秒前
5秒前
7秒前
8秒前
Chemistry发布了新的文献求助10
12秒前
Dingyiren发布了新的文献求助10
12秒前
wang完成签到,获得积分10
14秒前
Ecc完成签到,获得积分10
16秒前
洋溢完成签到,获得积分10
16秒前
20秒前
龟龟完成签到 ,获得积分10
21秒前
24秒前
涛哥来科研完成签到 ,获得积分10
26秒前
科研通AI2S应助应谷槐采纳,获得10
26秒前
29秒前
Konradling完成签到,获得积分10
33秒前
Ecc发布了新的文献求助10
36秒前
斯文败类应助缓慢天菱采纳,获得10
40秒前
49秒前
文献完成签到,获得积分10
49秒前
Seameng完成签到 ,获得积分10
58秒前
1分钟前
Demi_Ming完成签到,获得积分10
1分钟前
Chemistry发布了新的文献求助10
1分钟前
成就亦寒完成签到,获得积分20
1分钟前
1分钟前
1分钟前
hannuannuan发布了新的文献求助10
1分钟前
1分钟前
1分钟前
精明元霜完成签到,获得积分10
1分钟前
英勇羿发布了新的文献求助10
1分钟前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Foreign Policy of the French Second Empire: A Bibliography 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3146684
求助须知:如何正确求助?哪些是违规求助? 2798001
关于积分的说明 7826352
捐赠科研通 2454503
什么是DOI,文献DOI怎么找? 1306289
科研通“疑难数据库(出版商)”最低求助积分说明 627692
版权声明 601522