Natural polysaccharide based complex drug delivery system from microfluidic electrospray for wound healing

Natural polysaccharides have a demonstrated value in drug encapsulation and delivery. Efforts in this area are focused on improving the performances of these biomaterials. In this study, we present a multicompartment polysaccharide core-shell microparticle to construct a sustainable dual-release system of active molecules for enhancing wound healing. These microparticles were generated utilizing a microfluidic electrospray approach, in which a mixture of cellulose nanocrystals (CNC) and vascular endothelial growth factor (VEGF) were employed as the cores, with doxycycline hydrochloride (DH) mixed alginate as the shells. The resultant multicompartment particles exhibited wonderful antibacterial properties inherited from the alginate shell, and angiogenesis properties from the CNC core after the shell disintegrated. It was demonstrated that these core-shell microparticles performed their outstanding capabilities in inhibiting inflammation, promoting granulation tissue formation, collagen deposition, and angiogenesis, thereby accelerating rodents' wound healing. These findings support the great potential value of CNC/alginate core-shell microparticles based on the synergistic drug delivery strategy for orchestrating wound healing.