Exploring the regulatory mechanism of Nao Tai Fang on vascular Dementia's biological network based on cheminformatics and transcriptomics strategy

机制(生物学) 计算生物学 化学信息学 基因表达 医学 基因 生物信息学 生物 生物化学 认识论 哲学
作者
Zhao Da,Yi Yaqiao,Qi He,Shanshan Wang,Kailin Yang,Jinwen Ge
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:274: 114065-114065 被引量:14
标识
DOI:10.1016/j.jep.2021.114065
摘要

Nao Tai Fang (NTF) is modified from Buyang Huanwu Decoction. Modern pharmacological research showed that NTF has a good anti-cerebral ischemic effect and can improve the learning and memory ability of cerebrovascular disease. The purpose of this study is to explore the regulation mechanism of NTF on the regulation mechanism of vascular dementia (VD)'s biological network based on chemoinformatics and transcriptomics strategies. First, the bilateral common carotid artery ligation method was used to create a rat VD model. After NTF intervention for 30 days, the treatment effect was evaluated by HE staining and water maze experiment. Then, the Agilent mRNA expression profiling chip was used to obtain mRNA expression data of hippocampal tissues of VD model rats before and after NTF intervention, and microarray analysis was used to screen for genes with significant differential expression. The BATMAN database was utilized to obtain the potential targets of NTF and the Genecards and OMIM were utilized to collect the VD potential genes. The cytoscape was utilized to construct and analyze the networks. The animal experiments showed that NTF can improve VD. A total of 180 up-regulated proteins and 289 down-regulated proteins were identified. The top 20 up- and down-regulated differentially expressed genes were utilized to construct differentially expressed gene's protein-protein interaction (PPI) network. A total of 677 NTF potential targets and 550 VD genes were obtained and were utilized to construct NTF-VD PPI network. The cheminformatics analysis showed that NTF can regulate a lot of biological processes and signaling pathways (such as inflammation modules, vasodilation and contraction modules, hypoxia modules, angiogenesis, coagulation, neurovascular unit modules, Neuroactive ligand-receptor interaction, Calcium signaling pathway, Serotonergic synapse). NTF may play a role in the treatment of VD through the targets, signaling pathways and biological processes discovered in this study.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
令狐擎宇完成签到,获得积分10
刚刚
猪猪比特完成签到,获得积分10
刚刚
anyang完成签到,获得积分10
1秒前
冷酷的小玉完成签到 ,获得积分10
1秒前
风中的双完成签到 ,获得积分10
1秒前
ccc完成签到,获得积分10
1秒前
二硫碘化钾完成签到,获得积分10
3秒前
繁荣的代秋完成签到 ,获得积分10
3秒前
爽o完成签到 ,获得积分10
3秒前
小蘑菇应助Agamemnon采纳,获得10
4秒前
JamesPei应助糖豆豆吃豆豆采纳,获得10
5秒前
6秒前
吃饱了就晒太阳完成签到,获得积分10
6秒前
7秒前
zho发布了新的文献求助20
7秒前
小羊咩咩完成签到,获得积分10
7秒前
星辰大海应助伯爵采纳,获得10
9秒前
9秒前
a龙完成签到,获得积分10
9秒前
123完成签到,获得积分10
9秒前
2026毕业啦完成签到,获得积分10
9秒前
欧阳完成签到 ,获得积分20
9秒前
未来的幻想完成签到,获得积分10
10秒前
拾遗就是我完成签到,获得积分10
12秒前
WGOIST完成签到,获得积分10
14秒前
氘代乙腈是不贵的呀完成签到,获得积分10
14秒前
15秒前
沉静的红酒完成签到,获得积分10
15秒前
淡定的安白完成签到,获得积分10
15秒前
Haonan完成签到,获得积分10
16秒前
16秒前
Oliver完成签到,获得积分10
17秒前
HR112完成签到,获得积分10
17秒前
WWXWWX应助王京华采纳,获得10
18秒前
weeqe完成签到,获得积分10
19秒前
nine2652完成签到 ,获得积分10
19秒前
Karvs完成签到,获得积分10
19秒前
凳子琪完成签到,获得积分10
20秒前
唐唐的猫咪完成签到 ,获得积分10
20秒前
贱小贱完成签到,获得积分10
20秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 850
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248923
求助须知:如何正确求助?哪些是违规求助? 2892318
关于积分的说明 8270639
捐赠科研通 2560627
什么是DOI,文献DOI怎么找? 1389125
科研通“疑难数据库(出版商)”最低求助积分说明 651004
邀请新用户注册赠送积分活动 627855