A Proposed Method for Simultaneous Measurement of Cuticular Transpiration From Different Leaf Surfaces in Camellia sinensis

蒸腾作用 表皮蜡 表皮(毛发) 植物角质层 植物 生物 化学 光合作用 生物化学 遗传学
作者
Yi Zhang,Xiaobing Chen,Zhenghua Du,Wenjing Zhang,Ananta Raj Devkota,Zijian Chen,Changsong Chen,Weijiang Sun,Mingjie Chen
出处
期刊:Frontiers in Plant Science [Frontiers Media SA]
卷期号:11 被引量:15
标识
DOI:10.3389/fpls.2020.00420
摘要

The plant cuticle is the major barrier that limits unrestricted water loss and hence plays a critical role in plant drought tolerance. Due to the presence of stomata on the leaf abaxial surface, it is technically challenging to measure abaxial cuticular transpiration. Most of the existing reports were only focused on leaf astomatous adaxial surface, and few data are available regarding abaxial cuticular transpiration. Developing a method that can measure cuticular transpiration from both leaf surfaces simultaneously will improve our understanding about leaf transpiration barrier organization. Here, we developed a new method that enabled the simultaneous measurement of cuticular transpiration rates from the adaxial and abaxial surfaces. The proposed method combined multi-step leaf pretreatments including water equilibration under dark and ABA treatment to close stomata, as well as gum arabic or vaseline application to remove or seal the epicuticular wax layer. Mathematical formulas were established and used to calculate the transpiration rates of individual leaf surfaces from observed experimental data. This method facilitates the simultaneous quantification of cuticular transpiration from adaxial and abaxial leaf surfaces. By applying this method, we demonstrated that the adaxial intracuticular waxes and the abaxial epicuticular waxes constitute the major transpiration barriers in Camellia sinensis. Wax analysis indicated that adaxial intracuticular waxes had higher coverage of very long chain fatty acids, 1-alkanol esters, and glycols, which may be attributed to its higher transpiration barrier than that of the abaxial intracuticular waxes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
2秒前
3秒前
4秒前
loong完成签到,获得积分10
4秒前
CodeCraft应助rdd采纳,获得10
5秒前
5秒前
韫染发布了新的文献求助10
5秒前
8秒前
Arylkunst发布了新的文献求助10
8秒前
1234完成签到,获得积分20
8秒前
9秒前
9秒前
lxy56895发布了新的文献求助30
9秒前
曲珍完成签到,获得积分10
11秒前
小麻花发布了新的文献求助10
11秒前
12秒前
jtyuan完成签到,获得积分10
13秒前
CCD发布了新的文献求助10
14秒前
14秒前
Booksiy2发布了新的文献求助10
15秒前
乐陶陶完成签到,获得积分10
15秒前
吾身无拘发布了新的文献求助10
16秒前
17秒前
十指流玉完成签到,获得积分10
18秒前
18秒前
李健应助CCD采纳,获得10
19秒前
Arylkunst完成签到,获得积分10
19秒前
亮山火马完成签到,获得积分10
19秒前
下辈子不读书做只猪完成签到 ,获得积分10
19秒前
21秒前
王景完成签到,获得积分10
21秒前
韫染发布了新的文献求助10
21秒前
xxxxxxxxx发布了新的文献求助10
22秒前
23秒前
24秒前
雨雨发布了新的文献求助10
26秒前
隐形曼青应助zy采纳,获得10
27秒前
28秒前
11111发布了新的文献求助10
28秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3247880
求助须知:如何正确求助?哪些是违规求助? 2891121
关于积分的说明 8266211
捐赠科研通 2559325
什么是DOI,文献DOI怎么找? 1388116
科研通“疑难数据库(出版商)”最低求助积分说明 650698
邀请新用户注册赠送积分活动 627581