鼠李糖乳杆菌
副干酪乳杆菌
益生菌
生物
巨噬细胞极化
炎症
趋化因子
脂肪变性
微生物学
乳酸菌
免疫学
巨噬细胞
生物化学
内分泌学
细菌
体外
发酵
遗传学
作者
Luis Fontana,Julio Plaza‐Díaz,Paula Robles-Bolívar,Héctor Valente‐Godínez,María José Sáez‐Lara,Francisco Abadía‐Molina,Carolina Gómez‐Llorente,Ángel Gil,Ana I. Álvarez‐Mercado
出处
期刊:Nutrients
[MDPI AG]
日期:2021-01-11
卷期号:13 (1): 202-202
被引量:12
摘要
Non-alcoholic fatty liver disease (NAFLD) has reached pandemic proportions worldwide. We have previously reported that the probiotic strains Bifidobacterium breve CNCM I-4035, Lactobacillus paracasei CNCM I-4034 and Lactobacillus rhamnosus CNCM I-4036 exert anti-inflammatory effects in the intestine of Zucker-Leprfa/fa rats. In this work, we focused on their hepatic effects. M1 macrophages are related to inflammation and NAFLD pathogenesis, whereas M2 macrophages release anti-inflammatory mediators. We evaluated the effects of these 3 strains on macrophage polarization, inflammation and liver damage of Zucker-Leprfa/fa rats. The animals received either a placebo or 1010 CFU of probiotics orally for 30 days. Nos2 and Cd86 mRNA levels were determined as markers of M1 macrophages, and Cd163 and Arg1 as M2 markers, respectively, by qRT-PCR. Liver damage was determined by lipid peroxidation, leukocyte infiltration and myeloperoxidase activity. We evaluated a panoply of circulating chemokines, the hepatic ratio P-Akt/Akt, NF-kB and P-NF-kB protein levels. All 3 probiotic strains modulated macrophage polarization in liver and circulating levels of inflammation-related mediators. L. paracasei CNCM I-4034 increased the ratio P-Akt/Akt and NF-kB protein levels. B. breve CNCM I-4035, L. paracasei CNCM I-4034 and L. rhamnosus CNCM I-4036 decreased both pro-inflammatory macrophage gene expression and leukocyte infiltration in the liver.
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