抗菌肽
圆二色性
膜
化学
肽
作用机理
生物物理学
抗菌剂
拉曼光谱
组合化学
生物化学
生物
物理
有机化学
光学
体外
作者
Maria Giovanna Lizio,Mario Campana,Matteo De Poli,Damien Jefferies,Walter Cullen,Valery Andrushchenko,N.P. Chmel,Petr Bouř,Syma Khalid,Jonathan Clayden,Ewan W. Blanch,Alison Rodger,Simon J. Webb
出处
期刊:ChemBioChem
[Wiley]
日期:2021-01-11
卷期号:22 (9): 1656-1667
被引量:14
标识
DOI:10.1002/cbic.202000834
摘要
The increase in resistant bacterial strains necessitates the identification of new antimicrobial molecules. Antimicrobial peptides (AMPs) are an attractive option because of evidence that bacteria cannot easily develop resistance to AMPs. The peptaibols, a class of naturally occurring AMPs, have shown particular promise as antimicrobial drugs, but their development has been hindered by their mechanism of action not being clearly understood. To explore how peptaibols might interact with membranes, circular dichroism, vibrational circular dichroism, linear dichroism, Raman spectroscopy, Raman optical activity, neutron reflectivity and molecular dynamics simulations have been used to study a small library of peptaibol mimics, the Aib-rich peptides. All the peptides studied quickly partitioned and oriented in membranes, and we found evidence of chiral interactions between the phospholipids and membrane-embedded peptides. The protocols presented in this paper open new ground by showing how chiro-optical spectroscopies can throw light on the mechanism of action of AMPs.
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