活性氧
超氧化物歧化酶
生物膜
氧化应激
化学
聚赖氨酸
抗菌活性
食腐动物
抗生素
最小抑制浓度
IC50型
铜绿假单胞菌
细菌
体外
微生物学
抗氧化剂
生物化学
生物
遗传学
作者
Jingjing Zhang,Feng Qu,Taobo Liang,Sidi Lv,Wen Li,Qixiu You,Bolu Chen,Hengyi Xu
标识
DOI:10.1016/j.bej.2021.107937
摘要
Pseudomonas aeruginosa is one of the most commonly isolated nosocomial pathogens and is responsible for approximately 10 % of all hospital-acquired infections. P. aeruginosa is difficult to treat with known antibiotics due to its increasing antibiotic resistance. In our study, Ag NPs coated with ε-polylysine (ε-PL) and polyethyleneimine (PEI) were synthesized. These materials showed strong anti-P. aeruginosa capacity at an low concentration (IC50: 7.284 μg/mL). In addition, three drug-resistant clinical strains and biofilm formation were inhibited by ε-PL/PEI-coated Ag NPs (MBIC: 15.63 μg/mL). We further studied the antibacterial mechanism of Ag NPs on different levels and found that a) the amount of reactive oxygen species (ROS) increased, b) the expression of oxidative stress-related genes were up-regulated, c) the activity of total superoxide dismutase decreased, and d) the inhibitory rate is increased on P. aeruginosa cells. These antibacterial effects were dependent on an increasing concentration of Ag NPs but were neutralized by the ROS scavenger N-acetyl-L-cysteine. Therefore, we suggested that oxidative stress induced by ROS is the primary antibacterial mechanism of synthesized Ag NPs.
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