高致病性
动脉瘤
猪繁殖与呼吸综合征病毒
病毒学
生物
基因
多重聚合酶链反应
塔克曼
多路复用
拉伤
病毒
实时聚合酶链反应
聚合酶链反应
遗传学
传染病(医学专业)
疾病
H5N1亚型流感病毒
2019年冠状病毒病(COVID-19)
医学
病理
解剖
作者
Wenbin Qiu,Kai Meng,Yanyan Liu,Yuyu Zhang,Wang Zhao,Zhi Chen,Jie Yang,Wenbo Sun,Lihui Guo,Sufang Ren,Lei Chen,Yang Gao,Fan Zhang,Jianzhong Shi,Jun Li,Yijun Du,Jiang Yu,Jiaqiang Wu
标识
DOI:10.1016/j.jviromet.2019.113774
摘要
Porcine Reproductive and Respiratory Syndrome (PRRS), an acute infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is one of the most devastating diseases affecting the global swine industry. In order to establish a multiplex real-time PCR method for the simultaneous detection of the classical PRRSV (C-PRRSV) strain, the highly pathogenic PRRSV (HP-PRRSV) strain and NADC30-like PRRSV (NL-PRRSV) strain, we designed specific primers and TaqMan fluorescent probes based on the Nsp2 target gene sequence of these three different PRRSV strains, and designed American-type PRRSV (PRRSV-U) special primers and probes based on the relatively conserved target gene sequence of ORF7. The method established in this study can quickly and accurately detect and differentiate three types of strains of clinical tissue samples, respectively. This method plays a key role in the rapid diagnosis and determination of PRRSV.
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