[Expression of eosinophilic cationic protein in neutrophils and its effect on objective evaluation of nasal inflammation].

Objective:The aim of this study is to explore the expression of ECP in the neutrophils and its impact on the evaluation of nasal inflammation.Method:Neutrophils and eosinophils in nasal secretions were collected and stained with ECP immunohistochemistry to observe the staining of ECP in different cells. The concentration of ECP and MPO in nasal secretion were detected of 32 patients with allergic rhinitis (AR group), 29 patients with chronic rhinosinusitis without nasal polyps and allergic rhinitis (CRSsNP group), and 21 healthy people (control group). The percentage of neutrophils and eosinophils were calculated and analyzed as well.Result:ECP could be found in both eosinophils and neutrophils with immunohistochemical staining. The expression of ECP is much stronger in eosinophils than that in neutrophils. The ECP and MPO concentration in the nasal secretions of AR group and CRSsNP group were significantly higher than that in control group (P<0.000 1), and the ECP concentration in AR group and CRSsNP group had no difference. The expression of ECP in the AR group was not different from that in CRSsNP group, but the expression of MPO was significantly lower than that in CRSsNP group(P<0.000 1).Conclusion:ECP is expressed in neutrophils, and which is likely to have influence on the objective evaluation to nasal inflammation. Combining with the expression of ECP and MPO, we can make a more accurate judgment of local inflammation.目的：探索中性粒细胞中嗜酸粒细胞阳离子蛋白（ECP）的表达情况及其对鼻部炎症程度客观评价的影响。 方法：分别收集鼻分泌物中中性粒细胞及嗜酸粒细胞，进行ECP免疫组织化学染色，观察不同细胞中ECP的染色情况。选取40例变应性鼻炎（AR）患者为AR组，30例鼻分泌物以中性粒细胞表达为主、不伴有AR且不伴有鼻息肉的慢性鼻窦炎（CRSsNP）患者为CRSsNP组，24例正常人为对照组。收集3组对象的鼻分泌物，检测其中ECP、髓过氧化物酶（MPO）的含量，嗜酸粒细胞和中性粒细胞的数量及百分率，并进行分析。 结果：免疫组织化学染色后可见嗜酸粒细胞和中性粒细胞中均有ECP表达，嗜酸粒细胞中ECP的表达强度显著高于中性粒细胞。鼻分泌物炎症因子检测结果显示，AR组和CRSsNP组中ECP和MPO的表达水平均高于正常组（均P<0.000 1），AR组ECP表达水平与CRSsNP组无差异，但MPO表达水平显著低于CRSsNP组（P<0.000 1）。结论：中性粒细胞中有ECP表达，且可能对鼻部炎症客观评价造成影响。结合局部ECP及MPO的表达情况，可对局部炎症作出较为准确的判断。.