易位
生物
内膜
叶绿体膜
叶绿体
细胞生物学
膜蛋白
突变体
整体膜蛋白
跨膜蛋白
双精氨酸易位途径
生物发生
拟南芥
膜转运蛋白
遗传学
膜
类囊体
线粒体
基因
受体
作者
Yi-Shan Teng,Yi-shin Su,Lih‐Jen Chen,Yong Jik Lee,Inhwan Hwang,Hsou‐min Li
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2006-08-04
卷期号:18 (9): 2247-2257
被引量:160
标识
DOI:10.1105/tpc.106.044305
摘要
An Arabidopsis thaliana mutant defective in chloroplast protein import was isolated and the mutant locus, cia5, identified by map-based cloning. CIA5 is a 21-kD integral membrane protein in the chloroplast inner envelope membrane with four predicted transmembrane domains, similar to another potential chloroplast inner membrane protein-conducting channel, At Tic20, and the mitochondrial inner membrane counterparts Tim17, Tim22, and Tim23. cia5 null mutants were albino and accumulated unprocessed precursor proteins. cia5 mutant chloroplasts were normal in targeting and binding of precursors to the chloroplast surface but were defective in protein translocation across the inner envelope membrane. Expression levels of CIA5 were comparable to those of major translocon components, such as At Tic110 and At Toc75, except during germination, at which stage At Tic20 was expressed at its highest level. A double mutant of cia5 At tic20-I had the same phenotype as the At tic20-I single mutant, suggesting that CIA5 and At Tic20 function similarly in chloroplast biogenesis, with At Tic20 functioning earlier in development. We renamed CIA5 as Arabidopsis Tic21 (At Tic21) and propose that it functions as part of the inner membrane protein-conducting channel and may be more important for later stages of leaf development.
科研通智能强力驱动
Strongly Powered by AbleSci AI