十二指肠
普雷沃菌属
直肠
拟杆菌
生物
肠道菌群
粪便
厚壁菌
焦测序
微生物群
胃肠病学
活检
蛋白质细菌
微生物学
梭杆菌门
生理学
内科学
16S核糖体RNA
医学
细菌
免疫学
生物信息学
遗传学
基因
作者
Gangping Li,Min Yang,Kan Zhou,Lei Zhang,Lugao Tian,Shangze Lv,Yu Jin,Wei Qian,Hanhua Xiong,Rong Lin,Yu Fu,Xiaohua Hou
出处
期刊:Journal of Microbiology and Biotechnology
[Journal of Microbiology and Biotechnology]
日期:2015-07-28
卷期号:25 (7): 1136-1145
被引量:88
标识
DOI:10.4014/jmb.1412.12047
摘要
The diverse microbial communities that colonize distinct segments of the gastrointestinal tract are intimately related to aspects of physiology and the pathology of human health. However, most recent studies have focused on the rectal or fecal microbiota, and the microbial signature of the duodenum is poorly studied. In this study, we compared the microbiota in duodenal and rectal samples to illustrate the characteristic microbial signatures of the duodenum in healthy adults. Nine healthy volunteers donated biopsies and luminal contents from the duodenum and rectum. To determine the composition and diversity of the microbiota, 454- pyrosequencing of bacterial 16S rRNA was performed and multiple bioinformatics analyses were applied. The α-diversity and phylogenetic diversity of the microbiota in the duodenal samples were higher than those of the rectal samples. There was higher biodiversity among the microbiota isolated from rectal biopsies than feces. Proteobacteria were more highly represented in the duodenum than in the rectum, both in the biopsies and in the luminal contents from the healthy volunteers (38.7% versus 12.5%, 33.2% versus 5.0%, respectively). Acinetobacter and Prevotella were dominant in the duodenum, whereas Bacteroides and Prevotella were dominant in the rectum. Additionally, the percentage of OTUs shared in biopsy groups was far higher than in the luminal group (43.0% versus 26.8%) and a greater number of genera was shared among the biopsies than the luminal contents. Duodenal samples demonstrated greater biological diversity and possessed a unique microbial signature compared with the rectum. The mucosa-associated microbiota was more relatively conserved than luminal samples.
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