NLS公司
核定位序列
核运输
信号肽
转染
肽
生物
细胞生物学
计算生物学
基因
肽序列
细胞核
遗传学
生物化学
作者
Yanping Sun,Lei Xian,Haonan Xing,Jia Yu,Zhen Yang,Tianzhi Yang,Li Yang,Pingtian Ding
标识
DOI:10.1080/1061186x.2016.1184273
摘要
A nuclear localization signal (NLS) is a short amino acid sequence derived from eukaryotic nuclear proteins and viral proteins. Many NLS peptides can efficiently mediate the intranucleus transport of cargo molecules, so they have been widely used for non-viral gene transfer and shown potential ability to improve nuclear delivery of DNA. In order to maximally utilize NLS peptides to enhance gene transfer, several factors such as methods of incorporating NLS peptide, type and property of NLS peptide, number of NLS peptide, and spacer between NLS peptide and DNA should be considered. This review article summarizes how these factors influence the ability of NLS peptides in enhancing non-viral gene delivery and aids in defining the requirements for successful NLS-enhanced transfection.
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