等温滴定量热法
等温过程
量热法
化学
滴定法
动力学
酶动力学
热力学
化学动力学
化学反应
酶
物理化学
生物化学
活动站点
量子力学
物理
作者
Luca Mazzei,Stefano Ciurli,Barbara Zambelli
出处
期刊:Methods in Enzymology
日期:2016-01-01
卷期号:: 215-236
被引量:25
标识
DOI:10.1016/bs.mie.2015.07.022
摘要
Isothermal titration calorimetry (ITC) is a technique that measures the heat released or absorbed during a chemical reaction as an intrinsic probe to characterize any chemical process that involves heat changes spontaneously occurring during the reaction. The general features of this method to determine the kinetic and thermodynamic parameters of enzymatic reactions (kcat, KM, ΔH) are described and discussed here together with some detailed applications to specific cases. ITC does not require any modification or labeling of the system under analysis, can be performed in solution, and needs only small amounts of enzyme. These properties make ITC an invaluable, powerful, and unique tool to extend the knowledge of enzyme kinetics to drug discovery.
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