化学
ATP水解
心苷
ATP酶
酶
生物化学
体内
细胞内
磷酸盐
水解
膜
钠
色谱法
生物
哇巴因
有机化学
生物技术
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 89-104
被引量:5
标识
DOI:10.1007/978-1-4939-3179-8_10
摘要
The Na,K-ATPase is a plasma membrane enzyme that catalyzes active ion transport by the hydrolysis of ATP. Its activity in vivo is determined by many factors, particularly the concentration of intracellular sodium ions. It is the target of the cardiac glycoside class of drugs and of endogenous regulators. Its assay is often an endpoint in the investigation of physiological processes, and it is a promising drug target. As described in this unit, its enzymatic activity can be determined in extracts from tissues by test tube assay using a spectrophotometer or (32)P-ATP. The protocols in this chapter measure inorganic phosphate as the end product of hydrolysis of ATP.
科研通智能强力驱动
Strongly Powered by AbleSci AI