Wound Macrophages Express TGF-α and Other Growth Factors in Vivo: Analysis by mRNA Phenotyping

伤口愈合 生物 肉芽组织 生长因子 核糖核酸 信使核糖核酸 分子生物学 表皮生长因子 体内 转化生长因子 细胞生物学 转化生长因子-α 细胞生长 细胞培养 基因 免疫学 生物化学 遗传学 受体 生物技术
作者
Daniel A. Rappolee,Mark David,Michael J. Banda,Zena Werb
出处
期刊:Science [American Association for the Advancement of Science (AAAS)]
卷期号:241 (4866): 708-712 被引量:1104
标识
DOI:10.1126/science.3041594
摘要

The presence of macrophages is required for the regeneration of many cell types during wound healing. Macrophages have been reported to express a wide range of mitogenic factors and cytokines, but none of these factors has been shown in vivo to sustain all the wound-healing processes. It has been suggested that transforming growth factor-α (TGF-α) may mediate angiogenesis, epidermal regrowth, and formation of granulation tissue in vivo. Macrophages isolated from a wound site, and not exposed to cell culture conditions, expressed messenger RNA transcripts for TGF-α, TGF-β, platelet-derived growth factor A-chain, and insulin-like growth factor-1. The expression of these transcripts was determined by a novel method for RNA analysis in which low numbers of mouse macrophages were isolated from wound cylinders, their RNA was purified and reverse-transcribed, and the complementary DNA was amplified in a polymerase chain reaction primed with growth factor sequence-specific primers. This single-cell RNA phenotyping procedure is rapid and has the potential for quantification, and mRNA transcripts from a single cell or a few cells can be unambiguously demonstrated, with the simultaneous analysis of several mRNA species. Macrophages from wounds expressed TGF-α antigen, and wound fluids contained TGF-α. Elicited macrophages in culture also expressed TGF-α transcripts and polypeptide in a time-dependent manner after stimulation with modified low-density lipoproteins and lipopolysaccharide endotoxin, which are characteristic of the activators found in injured tissues.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
luoliping发布了新的文献求助10
1秒前
NSK发布了新的文献求助10
2秒前
2秒前
4秒前
鱼腩完成签到,获得积分10
4秒前
5秒前
yimuchenlin完成签到,获得积分10
5秒前
Jasper应助坤wkl采纳,获得10
5秒前
6秒前
paparazzi221应助王饱饱采纳,获得50
6秒前
6秒前
6秒前
sss发布了新的文献求助10
7秒前
8秒前
小二郎应助dongqing12311采纳,获得10
8秒前
buuyoo发布了新的文献求助10
9秒前
veronica发布了新的文献求助10
9秒前
10秒前
10秒前
10秒前
不懈奋进应助科研小白采纳,获得30
10秒前
s211998发布了新的文献求助10
11秒前
11秒前
Lucas发布了新的文献求助10
11秒前
科研通AI2S应助外向小刺猬采纳,获得10
12秒前
沉默惋庭完成签到,获得积分20
12秒前
Yidong发布了新的文献求助10
12秒前
Pericardium完成签到 ,获得积分10
13秒前
zz完成签到,获得积分10
14秒前
14秒前
搜集达人应助tom81882采纳,获得10
14秒前
15秒前
李无敌发布了新的文献求助10
16秒前
vg完成签到 ,获得积分10
16秒前
16秒前
谦让的大树完成签到,获得积分10
17秒前
调研昵称发布了新的文献求助10
18秒前
18秒前
坤wkl发布了新的文献求助10
18秒前
陈图图完成签到,获得积分10
19秒前
高分求助中
Earth System Geophysics 1000
Studies on the inheritance of some characters in rice Oryza sativa L 600
Medicina di laboratorio. Logica e patologia clinica 600
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Language injustice and social equity in EMI policies in China 500
mTOR signalling in RPGR-associated Retinitis Pigmentosa 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3206987
求助须知:如何正确求助?哪些是违规求助? 2856316
关于积分的说明 8104204
捐赠科研通 2521502
什么是DOI,文献DOI怎么找? 1354661
科研通“疑难数据库(出版商)”最低求助积分说明 642050
邀请新用户注册赠送积分活动 613292