This paper presents a theoretical study on a photonic crystal fiber (PCF) surface plasmon resonance biosensor. The proposed PCF sensor introduces the concept of simultaneous detection with H E 11x and H E 11x modes, which opens up some possibilities for multianalyte/multichannel sensing. Analysis was performed which considered the operation of the sensor in both amplitude and wavelength interrogation modes. Typical sensor resolutions of 4×10 -5 RIU and 8×10 -5 RIU with respect to H E 11x and H E 11y , respectively, are reported for the amplitude interrogation mode, while resoutions of 5 × 10 -5 RIU and 6×10 -5 RIU are reported for H E 11x and H E 11y , respectively, for the wavelength interrogation mode.