氧化应激
百草枯
神经保护
黑质
多巴胺能
药理学
活性氧
帕金森病
化学
抗氧化剂
神经毒性
体内
生物
生物化学
医学
神经科学
内科学
多巴胺
毒性
疾病
有机化学
生物技术
作者
Jui‐Ming Sun,Surbhi Agarwal,Tushar Dnyaneshwar Desai,Da‐Tong Ju,Yung‐Ming Chang,Shih‐Chieh Liao,Tsung‐Jung Ho,Yu‐Lan Yeh,Wei‐Wen Kuo,Yu‐Jung Lin,Chih‐Yang Huang
摘要
Parkinson's disease (PD) is a common neurodegenerative disorder associated with striatal dopaminergic neuronal loss in the Substantia nigra. Oxidative stress plays a significant role in several neurodegenerative diseases. Paraquat (PQ) is considered a potential neurotoxin that affects the brain leading to the death of dopaminergic neurons mimicking the PD phenotype. Various scientific reports have proven that cryptotanshinone possesses antioxidant and anti-inflammatory properties. We hypothesized that cryptotanshinone could extend its neuroprotective activity by exerting antioxidant effects. This study was designed to evaluate the effects of cryptotanshinone in both cellular and animal models of PQ-induced PD. Annexin V-PI double staining and immunoblotting were used to detect apoptosis and oxidative stress proteins, respectively. Reactive oxygen species kits were used to evaluate oxidative stress in cells. For in vivo studies, 18 B6 mice were divided into three groups. The rotarod data revealed the motor function and immunostaining showed the survival of TH+ neurons in SNpc region. Our study showed that cryptotanshinone attenuated paraquat-induced oxidative stress by upregulating anti-oxidant markers in vitro, and restored behavioral deficits and survival of dopaminergic neurons in vivo, demonstrating its therapeutic potential.
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