Laminated bamboo presents significant potential for applications in the construction industry. The stress-strain relationship and failure mechanism of laminated bamboo across the service temperature range, spanning from low to high temperatures, remain incompletely elucidated. Addressing this research gap, this paper conducts experiments on tension and compression of laminated bamboo in both parallel to grain direction and perpendicular to grain direction across an extensive temperature range of -40 to 80 °C. Temperature reduction factors according to Eurocode 5 are established, followed by adjustments based on RO constitutive model. Subsequently, a two-section constitutive model for laminated bamboo materials under service temperature is developed. This model is designed to accurately describe the stress-strain relationship of laminated bamboo in various temperature environments. The constitutive model proposed in this paper adeptly predicts the strain hardening behavior of laminated bamboo during compression, demonstrating high accuracy post-yield stress and addressing the non-convergence issue prevalent in most existing models. Additionally, the influence of temperature on fracture mechanism was explored using Scanning Electron Microscopy (SEM). The study delves deeply into the fundamental reasons behind temperature's effect on the mechanical properties of laminated bamboo, examining it from a chemical composition standpoint. This study not only offers significant insights for the application of laminated bamboo in construction projects but also serves as a valuable resource for advancing the understanding of laminated bamboo's mechanical behavior under various temperature conditions. The findings are crucial for architects, engineers, and material scientists, guiding them in optimizing the use of laminated bamboo in sustainable building practices and enhancing the structural integrity of bamboo-based construction.