生物正交化学
催化作用
膜
脂质体
纳米技术
化学
生物物理学
组合化学
材料科学
生物化学
生物
点击化学
作者
Yuanyuan Chen,Tong Wu,Shasha Xie,Yugang Bai,Hang Xing
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2023-05-10
卷期号:9 (19)
被引量:11
标识
DOI:10.1126/sciadv.adg2583
摘要
An obstacle to conducting diverse bioorthogonal reactions in living systems is the sensitivity of artificial metal catalysts. It has been reported that artificial metallocatalysts can be assembled in “cleaner” environments in cells for stabilized performance, which is powerful but is limited by the prerequisite of using specific cells. We report here a strategy to establish membrane-anchored catalysts with precise spatial control via liposome fusion–based transport (MAC-LiFT), loading bioorthogonal catalytic complexes onto either or both sides of the membrane leaflets. We show that the inner face of the cytoplasmic membrane serves as a reliable shelter for metal centers, protecting the complexes from deactivation thus substantially lowering the amount of catalyst needed for effective intracellular catalysis. This MAC-LiFT approach makes it possible to establish catalyst-protective systems with exclusively exogenous agents in a wide array of mammalian cells, allowing convenient and wider use of diverse bioorthogonal reactions in live cellular systems.
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