Expression and Characterization of Dichloromethane Dehalogenase for Dichloromethane Degradation

Dehalogenases widely used to remediate halocarbons contaminated soil. Genetic modification and over expression of recombinant dehalogenases will greatly facilitate its application in environment remediation area. In this study, a gene encoding dehalogenase (LinB) was modificated and over expressed in Pichia pastoris expression system. The results showed that the intracellular recombinant enzymatic activity were most active when the recombinant bacterium cultured at 30 ℃ for 4 h with glycerin 10 g/L as inducer, and the removal rate of 2-choloroethy ethyl sulphide by intracellular enzyme solution was 86.44%.