Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar

疫霉菌 生物 植物 蛋白质组 次生代谢 代谢组 代谢组学 生物化学 生物合成 生物信息学
作者
Martin Černý,Miroslav Berka,Miloň Dvořák,Ivan Milenković,Iñigo Saiz‐Fernández,Břetislav Brzobohatý,Jaroslav Ďurkovič
出处
期刊:Frontiers in Plant Science [Frontiers Media SA]
卷期号:13 被引量:4
标识
DOI:10.3389/fpls.2022.1018272
摘要

Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 × (Populus × canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
含蓄芷文完成签到,获得积分10
刚刚
高贵雁开发布了新的文献求助10
刚刚
水的叶子66完成签到,获得积分10
刚刚
iNk应助顺利琦采纳,获得10
刚刚
zhou完成签到,获得积分10
刚刚
Rikuya发布了新的文献求助10
刚刚
大个应助lemon采纳,获得10
1秒前
1秒前
ljh发布了新的文献求助10
2秒前
含蓄芷文发布了新的文献求助10
2秒前
4秒前
张张张完成签到,获得积分10
4秒前
万能图书馆应助图们江采纳,获得30
4秒前
蜀黍完成签到 ,获得积分10
5秒前
方糖完成签到,获得积分10
5秒前
Owen应助peng采纳,获得10
5秒前
好好好完成签到,获得积分10
5秒前
6秒前
6秒前
jackdawjo完成签到,获得积分10
7秒前
evan_L发布了新的文献求助10
7秒前
易小名完成签到 ,获得积分10
7秒前
蔡雨岑完成签到,获得积分10
7秒前
科研通AI2S应助科研通管家采纳,获得10
8秒前
CodeCraft应助科研通管家采纳,获得30
8秒前
priss111应助科研通管家采纳,获得30
8秒前
yy2023应助科研通管家采纳,获得10
8秒前
CodeCraft应助科研通管家采纳,获得10
8秒前
兜兜完成签到,获得积分10
8秒前
科研小桶完成签到,获得积分20
8秒前
Orange应助科研通管家采纳,获得10
8秒前
8秒前
pluto应助科研通管家采纳,获得10
8秒前
pluto应助科研通管家采纳,获得10
8秒前
小二郎应助科研通管家采纳,获得10
8秒前
orixero应助科研通管家采纳,获得10
8秒前
彭于晏应助科研通管家采纳,获得30
8秒前
8秒前
9秒前
hxw发布了新的文献求助10
9秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3245290
求助须知:如何正确求助?哪些是违规求助? 2888921
关于积分的说明 8256346
捐赠科研通 2557298
什么是DOI,文献DOI怎么找? 1385998
科研通“疑难数据库(出版商)”最低求助积分说明 650265
邀请新用户注册赠送积分活动 626527