线粒体分裂
癌变
癌症研究
细胞周期蛋白E1
细胞生物学
生物
分子生物学
激酶
线粒体
蛋白激酶A
癌症
细胞周期蛋白依赖激酶2
遗传学
作者
Saiyan Bian,Wenkai Ni,Linlin Zhou,Yun Tong,Chengchen Dai,Xuying Zhao,Yuwei Qiang,Jie Gao,Yina Xiao,Wei Liu,Changsheng Chen,Shengli Lin,Jianing Gong,Suming Zhao,Yinqi Chen,Zhaoyi Lin,Dong Liu,Hui Zhao,Wenjie Zheng
标识
DOI:10.1038/s41418-024-01342-1
摘要
Although deubiquitinases (DUBs) have been well described in liver tumorigenesis, their potential roles and mechanisms have not been fully understood. In this study, we identified ubiquitin-specific protease 1 (USP1) as an oncogene with essential roles during hepatocellular carcinoma (HCC) progression. USP1, with elevated expression levels and clinical significance, was identified as a hub DUB for HCC in multiple bioinformatics datasets. Functionally, USP1 overexpression significantly enhanced the malignant behaviors in HCC cell lines and spheroids in vitro, as well as the zebrafish model and the xenograft model in vivo. In contrast, genetic ablation or pharmacological inhibition of USP1 dramatically impaired the phenotypes of HCC cells. Specifically, ectopic USP1 enhanced aggressive properties and metabolic reprogramming of HCC cells by modulating mitochondrial dynamics. Mechanistically, USP1 induced mitochondrial fission by enhancing phosphorylation of Drp1 at Ser616 via deubiquitination and stabilization of cyclin-dependent kinase 5 (CDK5), which could be degraded by the E3 ligase NEDD4L. The USP1/CDK5 modulatory axis was activated in HCC tissues, which was correlated with poor prognosis of HCC patients. Furthermore, Prasugrel was identified as a candidate USP1 inhibitor for targeting the phenotypes of HCC by an extensive computational study combined with experimental validations. Taken together, USP1 induced malignant phenotypes and metabolic reprogramming by modulating mitochondrial dynamics in a CDK5-mediated Drp1 phosphorylation manner, thereby deteriorating HCC progression.
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