亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Downregulation of FHL2 suppressed trophoblast migration, invasion and epithelial–mesenchymal transition in recurrent miscarriage

滋养层 上皮-间质转换 基因敲除 细胞迁移 生物 波形蛋白 癌症研究 小干扰RNA 庆大霉素保护试验 下调和上调 免疫印迹 男科 转染 细胞 免疫学 胎盘 细胞培养 医学 胎儿 免疫组织化学 基因 怀孕 生物化学 遗传学
作者
Xiaorui Luan,Junyu Zhai,Shang Li,Yanzhi Du
出处
期刊:Reproductive Biomedicine Online [Elsevier BV]
卷期号:: 103342-103342
标识
DOI:10.1016/j.rbmo.2023.103342
摘要

Is four and a half LIM domain 2 (FHL2) involved in trophoblast migration, invasion and epithelial-mesenchymal transition (EMT) in recurrent miscarriage?Villus tissue was collected from 24 patients who had experienced recurrent miscarriage and 24 healthy controls. FHL2 mRNA and protein expression in villus specimens were observed by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Small interfering RNA and overexpression plasmid were used to change the FHL2 expression. JAR and HTR8/SVneo cell lines were used to conduct scratch-wound assay and transwell assay to detect trophoblast migration and invasion of FHL2. Downstream molecule expression of mRNA and protein and EMT markers were verified by qRT-PCR and Western blot.Significantly lower FHL2 mRNA (P = 0.019) and protein (P = 0.0014) expression was found in trophoblasts from the recurrent miscarriage group compared with healthy controls. FHL2 knockdown repressed migration (P = 0.0046), invasion (P < 0.001) and EMT, as shown by significant differences in mRNA and protein expression of the EMT markers N-cadherin, E-cadherin, Vimentin and Snail (all P < 0.05) of extravillus trophoblasts. FHL2 overexpression enhanced migration (P = 0.025), invasion (P < 0.001) and EMT of extravillus trophoblasts (all EMT markers P < 0.05). The positive upstream factor FHL2 in the extracellular signal-related kinase pathway induced JunD expression, thereby promoting trophoblast migration and invasion via matrix metalloproteinase 2.FHL2 is involved in a regulatory pathway of trophoblast migration, invasion and EMT during early pregnancy, and may have a role in recurrent miscarriage pathogenesis, which can serve as a possible target for novel therapeutic development.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
123321完成签到 ,获得积分10
2秒前
xiaoyan完成签到,获得积分10
4秒前
leonzhou发布了新的文献求助10
6秒前
aa发布了新的社区帖子
7秒前
郭志晟完成签到 ,获得积分10
11秒前
逮劳完成签到 ,获得积分10
12秒前
13秒前
sljzhangbiao11完成签到,获得积分10
18秒前
Freedom_1996完成签到,获得积分10
18秒前
yz_qi完成签到 ,获得积分10
24秒前
Bio应助sljzhangbiao11采纳,获得30
25秒前
mwm完成签到 ,获得积分10
32秒前
35秒前
我爱物理完成签到,获得积分10
36秒前
喜悦宫苴完成签到,获得积分10
39秒前
Z小姐完成签到 ,获得积分10
40秒前
合一海盗完成签到,获得积分10
43秒前
春山完成签到 ,获得积分10
44秒前
45秒前
科研通AI5应助科研通管家采纳,获得10
46秒前
脑洞疼应助科研通管家采纳,获得10
46秒前
46秒前
46秒前
47秒前
贪玩小小完成签到 ,获得积分10
50秒前
58秒前
学渣路过完成签到,获得积分0
1分钟前
satisusu完成签到 ,获得积分10
1分钟前
搜文献的北北完成签到,获得积分10
1分钟前
好巧完成签到,获得积分10
1分钟前
1分钟前
糯米完成签到 ,获得积分10
1分钟前
XING完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
喂喂发布了新的文献求助10
1分钟前
1分钟前
一颗滚石发布了新的文献求助20
1分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990012
求助须知:如何正确求助?哪些是违规求助? 3532047
关于积分的说明 11256141
捐赠科研通 3270918
什么是DOI,文献DOI怎么找? 1805105
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809216