终止因子
生物
细胞生物学
抄写(语言学)
核糖核酸
古细菌
一般转录因子
延伸系数
遗传学
RNA聚合酶
发起人
基因表达
基因
核糖体
语言学
哲学
作者
Chengyuan Wang,Vadim Molodtsov,Travis J. Sanders,Craig J. Marshall,Emre Firlar,Jason T. Kaelber,Thomas J. Santangelo,Richard H. Ebright
标识
DOI:10.1101/2023.08.09.552649
摘要
Abstract The ribonuclease FttA mediates factor-dependent transcription termination in archaea 1–3 . Here, we report the structure of a Thermococcus kodakarensis transcription pre-termination complex comprising FttA, Spt4, Spt5, and a transcription elongation complex (TEC). The structure shows that FttA interacts with the TEC in a manner that enables RNA to proceed directly from the TEC RNA-exit channel to the FttA catalytic center and that enables endonucleolytic cleavage of RNA by FttA, followed by 5’→3’ exonucleolytic cleavage of RNA by FttA and concomitant 5’→3’ translocation of FttA on RNA, to apply mechanical force to the TEC and trigger termination. The structure further reveals that Spt5 bridges FttA and the TEC, explaining how Spt5 stimulates FttA-dependent termination. The results reveal functional analogy between bacterial and archaeal factor-dependent termination, reveal functional homology between archaeal and eukaryotic factor-dependent termination, and reveal fundamental mechanistic similarities in factor-dependent termination in the three domains of life: bacterial, archaeal, and eukaryotic. One sentence summary Cryo-EM reveals the structure of the archaeal FttA pre-termination complex
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