蜂毒肽
前列腺癌
癌症研究
微阵列分析技术
生物
癌症
小桶
信号转导
基因表达
基因
细胞生物学
转录组
遗传学
膜
作者
Rucheng Yan,Weiwei Dai,Yiting Mao,Guopeng Yu,Wenfeng Li,Minfeng Shu,Bin Xu
出处
期刊:The Prostate
[Wiley]
日期:2023-07-30
卷期号:83 (15): 1430-1445
被引量:4
摘要
Abstract Background Melittin is a small molecule polypeptide extracted from the abdominal cavity of bees, which is used to treat inflammatory diseases and relieve pain. However, the antitumor effect of melittin and its mechanisms remain unclear, especially in castration‐resistant prostate cancer (CRPC). Methods Through CCK‐8 assay, colony formation assay, wound healing assay and Transwell migration assay, we explored the effect of melittin on CRPC cell lines. In addition, with microarray analysis, gene ontology analysis and kyoto encyclopedia of genes and genomes analysis, this study identified key genes and signaling pathways that influence the growth of PC‐3 cells. Meanwhile, the effect of melittin on CRPC was also verified through subcutaneous tumor formation experiments. Finally, we also tested the relevant indicators of human prostate cancer (PCa) specimens through immunohistochemistry and H&E stating. Results Here, melittin was verified to inhibit the cell proliferation and migration of CPRC. Moreover, RNA‐sequence analysis demonstrated that Interleukin‐17 (IL‐17) signaling pathway gene Lipocalin‐2 (LCN2) was downregulated by melittin treatment in CRPC. Further investigation revealed that overexpression of LCN2 was able to rescue tumor suppression and cisplatin sensitivity which melittin mediated. Interestingly, the expression of LCN2 is highly related to metastasis in PCa. Conclusions In brief, our study indicates that LCN2 plays an oncogenic role in CRPC and melittin may be selected as an attractive candidate for CRPC therapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI