已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Peroxisome Proliferator-Activated Receptor γ Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy

自噬 细胞生物学 脂质代谢 脂滴 PI3K/AKT/mTOR通路 溶酶体 过氧化物酶体增殖物激活受体 化学 受体 滋养层 罗格列酮 生物 信号转导 生物化学 细胞凋亡 胎盘 胎儿 怀孕 遗传学
作者
Kexing Hao,Jing Wang,Hengbin Yu,Lei Chen,Weibin Zeng,Wang Zheng-rong,Guangdong Hu
出处
期刊:Ppar Research [Hindawi Limited]
卷期号:2023: 1-13 被引量:9
标识
DOI:10.1155/2023/6422804
摘要

Peroxisome proliferator-activated receptor gamma (PPARγ) is a key nuclear receptor transcription factor that is highly expressed in trophoblastic cells during embryonic attachment and is accompanied by rapid cell proliferation and increased lipid accumulation. We previously showed that the autophagy pathway is activated in cells after activation of PPARγ, accompanied by increased lipid accumulation. In this study, we used PPARγ agonist rosiglitazone and inhibitor GW9662, as well as autophagy activator rapamycin and inhibitor 3-methyladenine, to unravel the probable mechanism of PPARγ engaged in lipid metabolism in sheep trophoblast cells (STCs). After 12 h, 24 h, and 48 h of drug treatment, the levels of autophagy-related proteins were detected by Western blot, the triglyceride content and MDA level of cells were detected by colorimetry, and the lipid droplets and lysosomes were localized by immunofluorescence. We found that PPARγ inhibited the activity of mammalian target of rapamycin (mTOR) pathway in STCs for a certain period of time, promoted the increase of autophagy and lysosome formation, and enhanced the accumulation of lipid droplets and triglycerides. Compared with cells whose PPARγ function is activated, blocking autophagy before activating PPARγ will hinder lipid accumulation in STCs. Pretreatment of cells with rapamycin promoted autophagy with results similar to rosiglitazone treatment, while inhibition of autophagy with 3-methyladenine reduced lysosome and lipid accumulation. Based on these observations, we conclude that PPARγ can induce autophagy by blocking the mTOR pathway, thereby promoting the accumulation of lipid droplets and lysosomal degradation, providing an energy basis for the rapid proliferation of trophoblast cells during embryo implantation. In brief, this study partially revealed the molecular regulatory mechanism of PPARγ, mTOR pathway, and autophagy on trophoblast cell lipid metabolism, which provides a theoretical basis for further exploring the functional regulatory network of trophoblast cells during the attachment of sheep embryos.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI

祝大家在新的一年里科研腾飞
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
kk完成签到,获得积分10
2秒前
甜美河马完成签到,获得积分10
2秒前
乐观期待完成签到,获得积分10
2秒前
甜美河马发布了新的文献求助10
6秒前
斯文败类应助ysxl采纳,获得10
7秒前
Merci完成签到,获得积分10
8秒前
8秒前
8秒前
9秒前
Orange应助ZEOMENJID采纳,获得10
9秒前
Piggy发布了新的文献求助10
11秒前
drift完成签到,获得积分10
11秒前
高大厉完成签到 ,获得积分10
13秒前
牧青发布了新的文献求助10
14秒前
一一一发布了新的文献求助10
15秒前
15秒前
迅速的幻雪完成签到 ,获得积分10
17秒前
四叶完成签到 ,获得积分10
20秒前
安东晨晨完成签到,获得积分10
24秒前
科研通AI6.2应助牧青采纳,获得30
26秒前
ysxl发布了新的文献求助10
28秒前
Orange应助atun采纳,获得10
29秒前
CodeCraft应助北极光采纳,获得10
29秒前
mimi完成签到,获得积分10
31秒前
jz完成签到,获得积分10
31秒前
Tree完成签到 ,获得积分10
37秒前
落寞飞烟完成签到,获得积分10
37秒前
科研通AI6.1应助迷人紫山采纳,获得10
38秒前
小黄完成签到 ,获得积分10
38秒前
无极微光应助大气的梨愁采纳,获得20
39秒前
40秒前
玉米完成签到 ,获得积分10
41秒前
45秒前
哈哈哈开开心心完成签到,获得积分10
46秒前
47秒前
牧青发布了新的文献求助30
51秒前
17876581310完成签到 ,获得积分10
53秒前
胡梦祥完成签到,获得积分10
54秒前
虞头星星发布了新的文献求助10
54秒前
dolabmu完成签到 ,获得积分10
54秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Signals, Systems, and Signal Processing 510
Discrete-Time Signals and Systems 510
《The Emergency Nursing High-Yield Guide》 (或简称为 Emergency Nursing High-Yield Essentials) 500
The Dance of Butch/Femme: The Complementarity and Autonomy of Lesbian Gender Identity 500
Differentiation Between Social Groups: Studies in the Social Psychology of Intergroup Relations 350
Investigating the correlations between point load strength index, uniaxial compressive strength and Brazilian tensile strength of sandstones. A case study of QwaQwa sandstone deposit 300
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 计算机科学 有机化学 物理 生物化学 纳米技术 复合材料 内科学 化学工程 人工智能 催化作用 遗传学 数学 基因 量子力学 物理化学
热门帖子
关注 科研通微信公众号,转发送积分 5886128
求助须知:如何正确求助?哪些是违规求助? 6623202
关于积分的说明 15704659
捐赠科研通 5006660
什么是DOI,文献DOI怎么找? 2697232
邀请新用户注册赠送积分活动 1641059
关于科研通互助平台的介绍 1595346