Eradication of ibuprofen and diclofenac via in situ synthesized and immobilized bacterial laccase to Cu-based metal organic framework

In this study, a simple in situ method was utilized for immobilization of laccase from Alcaligenes faecalis XF1. Protein loading of around 67.48 % was computed onto laccase@CuBTc. Immobilized laccase on CuBTc retained approximately 86 % activity after 14 days of storage at 4 °C. It was also observed that after 56 days of monitoring, residual activity was 32 % detected as a result of enzyme leaching. In comparison to free and immobilized laccase exhibited higher activity, stability and reusability. The characterization of the fabricated laccase@CuBTc was done using SEM, XRD and FT-IR. Laccase@Cu-BTc particles were used to degrade diclofenac (DCF) and Ibuprofen (IBF). In the absence of any mediator, laccase@CuBTc efficiently degraded around 95 % DCF within first 60 min of incubation and around 96 % IBF was degraded in 3 h. The findings showed that Laccase@CuBTc was a more stable, reusable, and storage-friendly method for removing pharmaceutical impurities such DCF and IBF.