亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis

甘草 沙棘 医学 化学 传统医学 胃肠病学 生物 内科学 病理 食品科学 替代医学
作者
Junmei Wang,Chunxiu Ren,Lingling Jin,Wuliji Batu
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:314: 116591-116591 被引量:21
标识
DOI:10.1016/j.jep.2023.116591
摘要

Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China. It is composed of Hippophae rhamnoides (berries, 30 g), Aucklandiae costus Falc. (dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch. (dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g). It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress. Past studies demonstrated that Seabuckthorn Wuwei Pulvis improved lung inflammation and chronic bronchitis in mice. However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model were established by exposure to lipopolysaccharide (LPS) and smoking. These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17). Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively. Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function. The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry. 16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung. The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
汉堡包应助舒服的觅夏采纳,获得10
1秒前
suicone完成签到,获得积分10
8秒前
zqq完成签到,获得积分0
16秒前
18秒前
归陌完成签到 ,获得积分10
18秒前
43秒前
mmyhn发布了新的文献求助10
47秒前
59秒前
Dave发布了新的文献求助10
1分钟前
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
1分钟前
wbs13521完成签到,获得积分0
1分钟前
2分钟前
儒雅致远发布了新的文献求助10
2分钟前
2分钟前
Hello应助儒雅致远采纳,获得10
2分钟前
正在获取昵称中...完成签到,获得积分10
2分钟前
2分钟前
爆米花应助xiongdi521采纳,获得10
2分钟前
3分钟前
xiongdi521发布了新的文献求助10
3分钟前
xiongdi521完成签到,获得积分10
3分钟前
mmyhn发布了新的文献求助10
3分钟前
3分钟前
4分钟前
Liiiiiiiiii发布了新的文献求助10
4分钟前
三水完成签到 ,获得积分20
4分钟前
小净完成签到 ,获得积分20
4分钟前
cccttt完成签到,获得积分10
4分钟前
mmyhn发布了新的文献求助10
4分钟前
Echo完成签到,获得积分10
4分钟前
无花果应助zzx采纳,获得10
4分钟前
可爱的香菇完成签到 ,获得积分10
4分钟前
5分钟前
dovejingling完成签到,获得积分10
5分钟前
lulu发布了新的文献求助20
5分钟前
Jasper应助科研通管家采纳,获得10
5分钟前
顾矜应助科研通管家采纳,获得10
5分钟前
5分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990045
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256354
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805146
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228