骨细胞
类有机物
间充质干细胞
成骨细胞
细胞生物学
生物医学工程
3D生物打印
材料科学
干细胞
化学
体外
组织工程
生物
医学
生物化学
作者
Jianhua Zhang,Julia Griesbach,Marsel Ganeyev,Anna‐Katharina Zehnder,Peng Zeng,Gian Nutal Schädli,Anke M. de Leeuw,Yuxiao Lai,Marina Rubert,Ralph Müller
出处
期刊:Biofabrication
[IOP Publishing]
日期:2022-05-26
卷期号:14 (3): 035018-035018
被引量:25
标识
DOI:10.1088/1758-5090/ac73b9
摘要
Mechanical loading has been shown to influence various osteogenic responses of bone-derived cells and bone formationin vivo. However, the influence of mechanical stimulation on the formation of bone organoidin vitrois not clearly understood. Here, three-dimensional (3D) bioprinted human mesenchymal stem cells-laden graphene oxide composite scaffolds were cultured in a novel cyclic-loading bioreactors for up to 56 d. Our results showed that mechanical loading from day 1 (ML01) significantly increased organoid mineral density, organoid stiffness, and osteoblast differentiation compared with non-loading and mechanical loading from day 21. Importantly, ML01 stimulated collagen I maturation, osteocyte differentiation, lacunar-canalicular network formation and YAP expression on day 56. These finding are the first to reveal that long-term mechanical loading is required for the formation of 3D bioprinted functional osteocyte bone organoids. Such 3D bone organoids may serve as a human-specific alternative to animal testing for the study of bone pathophysiology and drug screening.
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