Based on proteomics to explore the mechanism of mecobalamin promoting the repair of injured peripheral nerves

肌动蛋白细胞骨架 细胞骨架 髓鞘 肌动蛋白 轴突 坐骨神经 再生(生物学) 细胞生物学 周围神经损伤 免疫印迹 免疫荧光 生物 医学 解剖 内科学 细胞 免疫学 生物化学 中枢神经系统 抗体 基因
作者
Zongliang Xiong,Yao Wang,Xianglin Ma,Chong Zhou,Xiaowen Jiang,Wenhui Yu
出处
期刊:Canadian Journal of Physiology and Pharmacology [NRC Research Press]
卷期号:100 (6): 562-572 被引量:5
标识
DOI:10.1139/cjpp-2021-0692
摘要

Mecobalamin is commonly used in the adjuvant intervention of various peripheral nerve injuries. Actin cytoskeleton plays a role in the regeneration of myelin and axon. Therefore, the purpose of this study was to explore the possibility of mecobalamin regulating actin cytoskeleton in repairing nerve injury. In this study, a crush injury on the right sciatic nerve of two groups of rats (12 in each group) was established. The control group was only given normal saline (i.g.), and the intervention group was given mecobalamin 1 mg/kg (i.g.). The rats were sacrificed on 28th day and the injured nerves were collected for proteomics. The result shows that regulation of actin cytoskeleton pathway changed significantly. The expression of protein Vav1 was verified by Western blot and immunofluorescence. In the intervention group, the nerve fiber structure was complete, the axons were dense and symmetrical, and the myelin sheath was compact and uniform in thickness. The positive rate of myelin basic protein and βⅢ-tubulin was higher than that in the control group. The findings of the study show that mecobalamin regulates the actin cytoskeleton in the repair of nerve damage and upregulates Vav1 in the regulation of actin cytoskeleton pathway.
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