Serum-Free Production, Concentration and Purification of Recombinant Retroviruses

BioTechniquesVol. 23, No. 5 BenchmarksOpen AccessSerum-Free Production, Concentration and Purification of Recombinant RetrovirusesJurgen Seppen, Ryan J. Kimmel & William R.A. OsborneJurgen Seppen*Address correspondence to Jurgen Seppen, Department of Pediatrics, RR244, University of Washington School of Medicine, 1959 NE Pacific St., Box 356320, Seattle, WA 98195-6320, USA. Internet: E-mail Address: seppen@u.washington.eduUniversity of Washington School of Medicine, Seattle, WA, USA, Ryan J. KimmelUniversity of Washington School of Medicine, Seattle, WA, USA & William R.A. OsborneUniversity of Washington School of Medicine, Seattle, WA, USAPublished Online:7 Aug 2018https://doi.org/10.2144/97235bm04AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail "Serum-Free Production, Concentration and Purification of Recombinant Retroviruses." , 23(5), pp. 788–790FiguresReferencesRelatedDetailsCited ByAn Efficient Large-Scale Retroviral Transduction Method Involving Preloading the Vector into a RetroNectin-Coated Bag with Low-Temperature Shaking15 January 2014 | PLoS ONE, Vol. 9, No. 1Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical TrialsChemoprotection by Transfer of Resistance Genes24 February 2009Functional Assessment of the Engraftment Potential of Gammaretrovirus-Modified CD34 + Cells, Using a Short Serum-Free Transduction ProtocolHuman Gene Therapy, Vol. 17, No. 7Functional Assessment of the Engraftment Potential of Gammaretrovirus-Modified CD34+ Cells, Using a Short Serum-Free Transduction Protocol30 June 2006 | Human Gene Therapy, Vol. 0, No. 0Upscaling of lentiviral vector production by tangential flow filtration1 January 2005 | The Journal of Gene Medicine, Vol. 7, No. 10Isolated Hepatocyte Transplantation for Crigler-Najjar Syndrome Type 17 July 2017 | Cell Transplantation, Vol. 14, No. 2-3Long-Term Erythropoietin Gene Expression from Transduced Cells in Bioisolator DevicesHuman Gene Therapy, Vol. 14, No. 17Retroviral Transduction and Engraftment Ability of Primate Hematopoietic Progenitor and Stem Cells Transduced Under Serum-Free versus Serum-Containing ConditionsMolecular Therapy, Vol. 5, No. 3Concentration of viral vectors by co-precipitation with calcium phosphate1 January 2001 | The Journal of Gene Medicine, Vol. 3, No. 2Low-speed centrifugation of retroviral vectors absorbed to a particulate substrate: a highly effective means of enhancing retroviral titre25 May 2000 | Gene Therapy, Vol. 7, No. 11Brief Report: Retroviral Preparations Derived from PA317 Packaging Cells Contain Inhibitors That Copurify with Viral Particles and Are Devoid of Viral Vector RNAHuman Gene Therapy, Vol. 11, No. 5Improved Titers of Retroviral Vectors from the Human FLYRD18 Packaging Cell Line in Serum- and Protein-Free MediumHuman Gene Therapy, Vol. 10, No. 12 Vol. 23, No. 5 Follow us on social media for the latest updates Metrics Downloaded 74 times History Published online 7 August 2018 Published in print November 1997 Information© 2018 Author(s)PDF download