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Table S1. Experimental information and data on lactylation sites. (A) Primer, oligo, and sequence information, related to STAR Methods.

(B) p53 target level under increasing serum lactate, related to Figure S1.
(C) Lactylation(K)Sites_Control vs. lactate vs. siAARS1 vs. siAARS1 plus lactate in HeLa, related to Figure 2.
(D) Lactylation(K)Sites_Vector vs. OE HsAlaRS in HEK293T, related to Figure 2.
(E) Lactylation(K)Sites_Vector vs. OE EcAlaRS in HEK293T, related to Figure 2.
(F) Shared sites and proteins between HsAlaRS and EcAlaRS, related to Figure 2.
(G) Lactylation(K)Sites_Control vs. β-alanine in HeLa, related to Figure 2.
(H) Lactylation(K)Sites_Cell lysate incubated with lactate, ATP, and AlaRS, related to Figure 3.
(I) Lactylation-mimicking tumor variants of p53 K120 and K139, related to Figure 6.
(J) Patient cohort information, related to Figure 7

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