标题 |
Differential expression of the protooncogene bcl-2 in normal and osteoarthritic human articular cartilage
相关领域
软骨
流式细胞术
免疫组织化学
骨关节炎
软骨细胞
染色
信使核糖核酸
医学
病理
分子生物学
免疫学
生物
解剖
基因
生物化学
替代医学
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L Erlacher, R Maier, R Ullrich, H Kiener… - The Journal of …, 1995 - europepmc.org Objective To investigate the expression of bcl-2 in healthy and osteoarthritic articular cartilage at the transcriptional level and at the protein level. Methods bcl-2 mRNA was detected by reverse transcriptase/polymerase chain reaction. The expression of bcl-2 protein was studied using flow cytometry analysis with a monoclonal antibody against intracellular bcl-2 and by immunohistochemistry. Results mRNA for bcl-2 was detectable in chondrocytes of both healthy and osteoarthritic cartilage. bcl-2 protein was present |
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