Total RNA was isolated from cultured cells and enteroids and colonoids, and homogenized colon tissues, using an RNA isolation kit (Qiagen, Valencia, CA) and was treated with RNase-free DNase. Reverse transcription was performed using the High Capacity cDNA Reverse Transcription kit and the 7300 RT-PCR System (Applied Biosystems, Foster City, CA). The data were analyzed using Sequence Detection System V1.4.0 software. Primers, Setd1b (Mm00616971), Tgfb1 (Mm01178820), TNF (Mm00443259), and Ifng (Mm99999071) were purchased from Applied Biosystems. The relative abundance of β-actin mRNA was used to normalize levels of the mRNAs of interest. All complementary DNA samples were analyzed in triplicate.
想请教文中的Mm00616971等序列如何查询。
拜托各位！
PDF的下载单位、IP信息已删除
(2025-6-4)
进进
无极微光
带头大哥
BowieHuang
蓝天
轨迹
小青椒
Criminology34
jiaaaaa
殷勤的紫槐
pluto
yznfly
闪闪的诗珊
机灵柚子
null
GingerF
old赵
RC_Wang
smottom
Twonej
niNe3YUE
Zhi
寻道图强
lizishu
皖公网安备34019202002308
