Decoding the dynamics of enhanced succinoglycan biosynthesis from pretreated sugarcane molasses using Rhizobium pusense KM7: Kinetics and structural insights

In the current investigation, an extensive investigation into the microbial synthesis of exopolysaccharides (EPS), with a focus on succinoglycan, was conducted using Rhizobium pusense KM7. Sugarcane molasses, a byproduct of agricultural industry, was harnessed as a substrate, underscoring its potential in waste-to-value conversions. By implementing response surface methodology, conditions were optimized for maximal EPS production, yielding a notable output of 21 g/L. The process was successfully scaled up to a 1L shake flask and a 5L bioreactor, with unstructured models utilized to decode the complex dynamics of microbial growth, substrate consumption, and product formation. Advanced techniques such as HPLC, GPC, TGA and FTIR analyses were employed to confirm the high-molecular-weight, heteropolymeric nature of the produced succinoglycan, as well as its substantial thermal stability. Our research heralds a promising future for sustainable, large-scale bio-production, harnessing agricultural waste and opening new avenues in the field of microbial biotechnology. The novelty of this study lies in the exploration of the kinetics of succinoglycan biosynthesis by Rhizobium pusense using cane molasses at a bioreactor scale, representing the first-ever report of this nature.