封堵器
腹泻
人参
PI3K/AKT/mTOR通路
蛋白激酶B
肠道菌群
促炎细胞因子
紧密连接
肿瘤坏死因子α
粘蛋白
肠易激综合征
医学
药理学
免疫学
微生物学
化学
生物
内科学
信号转导
炎症
生物化学
病理
替代医学
作者
Mengjie Zhao,Qiong Zhao,Zhiwei Guan,Qianwei Liu,Hongyun Zhou,Qinwan Huang,Bixiu Huo
出处
期刊:Journal of Medicinal Food
[Mary Ann Liebert]
日期:2023-02-24
卷期号:26 (3): 165-175
被引量:6
标识
DOI:10.1089/jmf.2022.k.0069
摘要
Panax ginseng and Fructus mume (Renshen Wumei in Chinese, RW) are natural medicines with high nutritional and pharmacological value. They have been widely used together in China to treat gastrointestinal diseases, especially persistent diarrhea, but the potential mechanisms remain elusive. In this study, a diarrhea model was established in rats using a 30% aqueous extract of senna. The therapeutic effects of RW were evaluated by recording the prevalence of loose stools, the diarrhea index, and histopathological changes in colon tissue. The levels of mucins, tight junction (TJ) proteins, inflammatory cytokines, and phosphoinositide 3-kinase/Akt/nuclear factor-κB (PI3K/Akt/NF-κB) signaling pathway proteins were measured. Metagenomic sequencing was used to analyze the gut microbiota. Treatment with RW alleviated injury to the intestinal barrier in rats with diarrhea and also upregulated levels of Muc2 and TJ proteins, such as occludin, zonula occludens-1, and claudin-1. Administration of RW regulated the structure of the gut microbiota in diarrheal rats. Furthermore, RW suppressed levels of interleukin (IL), tumor necrosis factor (TNF)-α, IL-1, PI3K, Akt, and p-NF-κB p65 and also increased IL-4 levels. Our study indicates that P. ginseng and Fructus mume help improve the symptoms of diarrhea, possibly by alleviating the intestinal barrier injury, regulating intestinal flora composition, and inhibiting the PI3K/Akt/NF-κB signaling pathway.
科研通智能强力驱动
Strongly Powered by AbleSci AI