劈开
核酸外切酶
DNA
核酸外切酶 III
化学
生物物理学
生物传感器
基质(水族馆)
组合化学
生物化学
生物
基因
大肠杆菌
DNA聚合酶
生态学
作者
Yi Shen,Haoyu Yuan,Zixuan Guo,Xiuqing Li,Zhiqing Yang,Chengli Zong
出处
期刊:Biosensors
[Multidisciplinary Digital Publishing Institute]
日期:2023-05-26
卷期号:13 (6): 581-581
摘要
Exonuclease III (Exo III) has been generally used as a double-stranded DNA (dsDNA)-specific exonuclease that does not degrade single-stranded DNA (ssDNA). Here, we demonstrate that Exo III at concentrations above 0.1 unit/μL can efficiently digest linear ssDNA. Moreover, the dsDNA specificity of Exo III is the foundation of many DNA target recycling amplification (TRA) assays. We demonstrate that with 0.3 and 0.5 unit/μL Exo III, the degradation of an ssDNA probe, free or fixed on a solid surface, was not discernibly different, regardless of the presence or absence of target ssDNA, indicating that Exo III concentration is critical in TRA assays. The study has expanded the Exo III substrate scope from dsDNA to both dsDNA and ssDNA, which will reshape its experimental applications.
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